Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI23353
Trapped Gene
Hnrpab (ENSMUSG00000020358)
Vector Insertion
Chr 11: 51419132 - 51419841
Public Clones IST14933F11 (tigm)
Private Clones OST262576 (lexicon)
Severity of mutation (?) Insertion after 24% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000722048 (Chr11:51419842..51420088 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000722048 (Chr11:51419842..51420088 -)
Downstram Exon
ENSMUSE00000103710 (Chr11:51418963..51419131 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000294713 Chr11:51420169..51420349 No primer for this exon
upstream ENSMUSE00000579971 Chr11:51419842..51420088 No primer for this exon
upstream ENSMUSE00000722048 Chr11:51419842..51420088 No primer for this exon

*** Putative Vector Insertion (Chr 11: 51419132 - 51419841) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000103710 Chr11:51418963..51419131 No primer for this exon
downstream ENSMUSE00000103709 Chr11:51418161..51418319 No primer for this exon
downstream ENSMUSE00000103708 Chr11:51417902..51418033 No primer for this exon
downstream ENSMUSE00000400204 Chr11:51416095..51416197 No primer for this exon
downstream ENSMUSE00000653762 Chr11:51415252..51415392 No primer for this exon
downstream ENSMUSE00000653761 Chr11:51414371..51415392 No primer for this exon
downstream ENSMUSE00000465513 Chr11:51413602..51414980 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGGCCGTCTTTTGTTGTAAT Chr11:51419786..51419806 58.95 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GTCTTTTGTTGCGTGACTGG Chr11:51419781..51419801 59.34 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 GGCTGAGGAGCAGCCTAATC Chr11:51420033..51420053 61.4 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector

Come back to gene ENSMUSG00000020358