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About
UniTrap UNI23377
Trapped Gene
l7Rn6 (
ENSMUSG00000062797
)
Vector Insertion
Chr 7: 97072073 - 97072226
Public Clones
not available
Private Clones
OST261754 (lexicon)
OST176883 (lexicon)
OST174966 (lexicon)
OST155566 (lexicon)
OST78831 (lexicon)
OST55235 (lexicon)
Severity of mutation
(?)
Insertion after 0% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000487322 (Chr7:97072074..97072225 -)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
CCTCCGTTGACTCCTTTACG
Chr7:97072077..97072096
59.73
55
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000487322 (Chr7:97072074..97072225 -)
Downstram Exon
ENSMUSE00000713158 (Chr7:97072074..97072225 -)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
CCTCCGTTGACTCCTTTACG
Chr7:97072077..97072096
59.73
55
AAAGGAGTCAACGGAGGACA
Chr7:97072058..97072077
59.7
50
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000489415
Chr7:97089479..97089695
TAGTGGAGGGTCACGTTCCT
Chr7:97089619..97089638
59.57
55
upstream
ENSMUSE00000672472
Chr7:97089479..97089594
No primer for this exon
upstream
ENSMUSE00000713514
Chr7:97089479..97089709
GAGGCGTGCAAGTTAGGAAG
Chr7:97089685..97089704
60.01
55
upstream
ENSMUSE00000672471
Chr7:97088490..97088553
GGTTCCAAGAAACCATGGAC
Chr7:97088524..97088543
59.24
50
upstream
ENSMUSE00000477314
Chr7:97084237..97084474
GGCATGGGAGGATCTGTCTA
Chr7:97084341..97084360
60.03
55
upstream
ENSMUSE00000487322
Chr7:97072074..97072225
CCTCCGTTGACTCCTTTACG
Chr7:97072077..97072096
59.73
55
upstream
ENSMUSE00000713158
Chr7:97072074..97072225
CCTCCGTTGACTCCTTTACG
Chr7:97072077..97072096
59.73
55
*** Putative Vector Insertion (Chr 7: 97072073 - 97072226) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000490457
Chr7:97068583..97068701
TGCTGGGATGAACATTTCTG
Chr7:97068578..97068597
59.65
45
downstream
ENSMUSE00000711507
Chr7:97067434..97067517
TTTCCAAAAGAGGGGGTTCT
Chr7:97067447..97067466
59.91
45
downstream
ENSMUSE00000672470
Chr7:97067430..97067517
TTTCCAAAAGAGGGGGTTCT
Chr7:97067447..97067466
59.91
45
downstream
ENSMUSE00000515882
Chr7:97067199..97067517
TTTCCAAAAGAGGGGGTTCT
Chr7:97067447..97067466
59.91
45
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
No PCR available
Come back to gene
ENSMUSG00000062797