Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI23571
Trapped Gene
Zfp628 (ENSMUSG00000074406)
Vector Insertion
Chr 7: 4867008 - 4869011
Public Clones (sanger)
Private Clones OST252808 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000705975 (Chr7:4866819..4867007 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000705975 (Chr7:4866819..4867007 +)
Downstram Exon
ENSMUSE00000705974 (Chr7:4869012..4869118 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon TGGCTGACTCTCCCTTCTGT Chr7:4869069..4869088 59.99 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000705975 Chr7:4866819..4867007 No primer for this exon

*** Putative Vector Insertion (Chr 7: 4867008 - 4869011) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000705974 Chr7:4869012..4869118 TGGCTGACTCTCCCTTCTGT Chr7:4869069..4869088 59.99 55
downstream ENSMUSE00000705973 Chr7:4870378..4873604 AGTTGTACCGTGGCCACTTC Chr7:4872856..4872875 60.03 55
downstream ENSMUSE00000637495 Chr7:4870404..4871195 TTTATAGGGCCGTTCACCTG Chr7:4870574..4870593 59.95 50
downstream ENSMUSE00000637494 Chr7:4871407..4871415 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GTTTAATCGCCTTGCAGCAC Chr7:4867056..4867076 60.78 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGTCGTTCGTGACTGGGAAA Chr7:4867052..4867072 60.69 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000074406