Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI23625
Trapped Gene
Tmem107 (ENSMUSG00000020895)
Vector Insertion
Chr 11: 68884794 - 68884875
Public Clones not available
Private Clones OST251129 (lexicon)
Severity of mutation (?) Insertion after 36% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000111984 (Chr11:68884726..68884793 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000111984 (Chr11:68884726..68884793 +)
Downstram Exon
ENSMUSE00000111987 (Chr11:68884876..68884976 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000414589 Chr11:68884311..68884535 No primer for this exon
upstream ENSMUSE00000588786 Chr11:68884314..68884535 No primer for this exon
upstream ENSMUSE00000111984 Chr11:68884726..68884793 No primer for this exon

*** Putative Vector Insertion (Chr 11: 68884794 - 68884875) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000111987 Chr11:68884876..68884976 No primer for this exon
downstream ENSMUSE00000466325 Chr11:68885925..68886021 No primer for this exon
downstream ENSMUSE00000588785 Chr11:68885925..68886794 No primer for this exon
downstream ENSMUSE00000588787 Chr11:68886248..68886528 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CGAAAAGCAGGACAACCAGT Chr11:68884777..68884797 60.29 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CGAAAAGCAGGACAACCAGT Chr11:68884777..68884797 60.29 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000020895