Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI2366
Trapped Gene
Baz2a (ENSMUSG00000040054)
Vector Insertion
Chr 10: 127562702 - 127563073
Public Clones AD0392 (sanger) AC0515 (sanger)
Private Clones not available
Severity of mutation (?) Insertion after 91% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000272638 (Chr10:127562555..127562701 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TTTCTCCTGCTGTGTGATGG Chr10:127562591..127562610 59.83 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000272638 (Chr10:127562555..127562701 +)
Downstram Exon
ENSMUSE00000272633 (Chr10:127563074..127563297 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TTTCTCCTGCTGTGTGATGG Chr10:127562591..127562610 59.83 50 TGGACTATCTCGGCTCCTTG Chr10:127563208..127563227 60.35 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000665442 Chr10:127496046..127496082 No primer for this exon
upstream ENSMUSE00000665441 Chr10:127533493..127533579 GGATTCTCTCCAACGACCAA Chr10:127533494..127533513 60.05 50
upstream ENSMUSE00000629097 Chr10:127545950..127546087 GGAGGCAAACGACCATTTTA Chr10:127545954..127545973 59.94 45
upstream ENSMUSE00000272816 Chr10:127547811..127548404 CATCCTGATGAAGCAGCAGA Chr10:127548308..127548327 60.1 50
upstream ENSMUSE00000272811 Chr10:127549638..127549823 TGGAGCCCTTTGACTCTCTG Chr10:127549797..127549816 60.52 55
upstream ENSMUSE00000272806 Chr10:127550519..127550737 TAGATGATGCGGAGCTGATG Chr10:127550546..127550565 59.93 50
upstream ENSMUSE00000272801 Chr10:127551390..127551839 AAGAGACGACTGGAGGAGCA Chr10:127551801..127551820 60.13 55
upstream ENSMUSE00000272794 Chr10:127552081..127552141 AAGTTCGTCTTCCCCTCCAG Chr10:127552117..127552136 60.62 55
upstream ENSMUSE00000272786 Chr10:127553117..127553225 AGAAGTGCGCATCAAGAAGG Chr10:127553126..127553145 60.54 50
upstream ENSMUSE00000272778 Chr10:127553362..127553463 TACCTGAGCCGAAATGTGGT Chr10:127553362..127553381 60.52 50
upstream ENSMUSE00000471272 Chr10:127553603..127553813 TGCAGTGGGTCCAGTTATCA Chr10:127553607..127553626 60.11 50
upstream ENSMUSE00000272762 Chr10:127555611..127555711 GATGAGGCAGAAGGTCCAAC Chr10:127555660..127555679 59.66 55
upstream ENSMUSE00000272755 Chr10:127556011..127556076 GGAAGCAAGACACCAAGAGC Chr10:127556024..127556043 60 55
upstream ENSMUSE00000272747 Chr10:127556326..127556556 AGGCCATCCTGTAGAGCAGA Chr10:127556431..127556450 59.97 55
upstream ENSMUSE00000272740 Chr10:127556680..127556934 ACTCCATGATCCTGGTCTGC Chr10:127556901..127556920 60.08 55
upstream ENSMUSE00000272733 Chr10:127558167..127558381 GAGATACTGCGCTGCTTCCT Chr10:127558227..127558246 59.75 55
upstream ENSMUSE00000272723 Chr10:127558647..127558721 GCTGCAGGAAGAACAAGTGG Chr10:127558679..127558698 60.97 55
upstream ENSMUSE00000272717 Chr10:127558856..127559036 GTTCTCGGATCATGGAGGAA Chr10:127558939..127558958 60.01 50
upstream ENSMUSE00000272710 Chr10:127559124..127559183 GTTGCAGCATCTAGCATTCC Chr10:127559130..127559149 58.48 50
upstream ENSMUSE00000272698 Chr10:127559362..127559506 CTTTGTGGAAGGATCGGAAG Chr10:127559478..127559497 59.67 50
upstream ENSMUSE00000272690 Chr10:127560080..127560711 CTCCGTCTCAGTCCTTGGAG Chr10:127560533..127560552 59.98 60
upstream ENSMUSE00000272684 Chr10:127560822..127561044 GGAGACCCCCTAGCAAGTTC Chr10:127560967..127560986 60.07 60
upstream ENSMUSE00000272674 Chr10:127561163..127561318 AAGGCGCTTCACAAACATCT Chr10:127561249..127561268 59.88 45
upstream ENSMUSE00000272667 Chr10:127561391..127561542 GAAGGCGTTATGAGCTGGTC Chr10:127561429..127561448 59.84 55
upstream ENSMUSE00000272659 Chr10:127561949..127562238 CCCTACTGAGCACACCCAAT Chr10:127562190..127562209 59.99 55
upstream ENSMUSE00000272646 Chr10:127562325..127562457 ATGAGATCACCCCTCGTGTC Chr10:127562330..127562349 59.93 55
upstream ENSMUSE00000272638 Chr10:127562555..127562701 TTTCTCCTGCTGTGTGATGG Chr10:127562591..127562610 59.83 50

*** Putative Vector Insertion (Chr 10: 127562702 - 127563073) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000272633 Chr10:127563074..127563297 TGGACTATCTCGGCTCCTTG Chr10:127563208..127563227 60.35 55
downstream ENSMUSE00000272627 Chr10:127563382..127563525 CATCATGGGACTCCATCTCC Chr10:127563416..127563435 60.29 55
downstream ENSMUSE00000395665 Chr10:127563644..127566359 CCTGCTGAAAAGATTGCACA Chr10:127565019..127565038 59.99 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CTGCTCTCCCATCTCCATTC Chr10:127562712..127562732 59.76 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CTGCTCTCCCATCTCCATTC Chr10:127562712..127562732 59.76 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000040054