Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI23681
Trapped Gene
Rps10 (ENSMUSG00000052146)
Vector Insertion
Chr 17: 27767366 - 27767452
Public Clones not available
Private Clones OST249312 (lexicon) OST248309 (lexicon)
Severity of mutation (?) Insertion after 92% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000139784 (Chr17:27767374..27767451 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CCACCTCAGTGAAGTTGGAGTT Chr17:27767400..27767421 60.58 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000139784 (Chr17:27767374..27767451 -)
Downstram Exon
ENSMUSE00000700898 (Chr17:27767367..27767451 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CCACCTCAGTGAAGTTGGAGTT Chr17:27767400..27767421 60.58 50 AACTTCACTGAGGTGGCTGA Chr17:27767384..27767403 58.44 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000700896 Chr17:27773510..27773574 CAGTCCCCCATCAGACAACT Chr17:27773521..27773540 59.96 55
upstream ENSMUSE00000700904 Chr17:27772149..27772221 No primer for this exon
upstream ENSMUSE00000700926 Chr17:27772149..27772185 No primer for this exon
upstream ENSMUSE00000549878 Chr17:27771271..27771420 CGGATTGCCATCTACGAACT Chr17:27771380..27771399 60.1 50
upstream ENSMUSE00000721935 Chr17:27771271..27771420 CGGATTGCCATCTACGAACT Chr17:27771380..27771399 60.1 50
upstream ENSMUSE00000139792 Chr17:27771017..27771188 CTCCGAGACTACCTGCACCT Chr17:27771085..27771104 59.47 60
upstream ENSMUSE00000139788 Chr17:27769224..27769301 ACCTACAGAAGGAGCGCTGT Chr17:27769229..27769248 59.12 55
upstream ENSMUSE00000700902 Chr17:27768145..27768200 GCTCAGCCACTGAGTTCCAG Chr17:27768148..27768167 61.15 60
upstream ENSMUSE00000139784 Chr17:27767374..27767451 CCACCTCAGTGAAGTTGGAGTT Chr17:27767400..27767421 60.58 50
upstream ENSMUSE00000700895 Chr17:27767369..27767451 CCACCTCAGTGAAGTTGGAGT Chr17:27767401..27767421 59.21 52.38
upstream ENSMUSE00000700898 Chr17:27767367..27767451 CCACCTCAGTGAAGTTGGAGT Chr17:27767401..27767421 59.21 52.38

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GTTGGATAATCGCCTTGCAG Chr17:27767387..27767407 60.61 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CCTCAGTGAAGTTGGACGTG Chr17:27767397..27767417 59.31 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000052146