Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI23732
Trapped Gene
Tmem107 (ENSMUSG00000020895)
Vector Insertion
Chr 11: 68886022 - 68886247
Public Clones IST14176H5 (tigm) IST12051A5 (tigm) IST13842H4 (tigm)
Private Clones OST246561 (lexicon)
Severity of mutation (?) Insertion after 84% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000466325 (Chr11:68885925..68886021 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000466325 (Chr11:68885925..68886021 +)
Downstram Exon
ENSMUSE00000588787 (Chr11:68886248..68886528 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000414589 Chr11:68884311..68884535 No primer for this exon
upstream ENSMUSE00000588786 Chr11:68884314..68884535 No primer for this exon
upstream ENSMUSE00000111984 Chr11:68884726..68884793 No primer for this exon
upstream ENSMUSE00000111987 Chr11:68884876..68884976 No primer for this exon
upstream ENSMUSE00000466325 Chr11:68885925..68886021 No primer for this exon
upstream ENSMUSE00000588785 Chr11:68885925..68886794 No primer for this exon

*** Putative Vector Insertion (Chr 11: 68886022 - 68886247) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000588787 Chr11:68886248..68886528 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ATAATCGCCTTGCAGCACAT Chr11:68886072..68886092 60.64 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AAATCGACGTGACTGGGAAA Chr11:68886066..68886086 60.49 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000020895