Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI23747
Trapped Gene
Zfyve28 (ENSMUSG00000037224)
Vector Insertion
Chr 5: 34576044 - 34576933
Public Clones not available
Private Clones OST246182 (lexicon) OST236510 (lexicon) OST139158 (lexicon)
Severity of mutation (?) Insertion after 19% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000600724 (Chr5:34576934..34577136 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GCACTCCGAGACCTCAACAC Chr5:34577000..34577019 60.87 60 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000600724 (Chr5:34576934..34577136 -)
Downstram Exon
ENSMUSE00000600723 (Chr5:34575954..34576043 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GCACTCCGAGACCTCAACAC Chr5:34577000..34577019 60.87 60 GAACAGCACGATGACCTCCT Chr5:34575949..34575968 60.27 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000242650 Chr5:34630748..34630975 CAGATTCCGAAAGTGGCTGT Chr5:34630759..34630778 60.26 50
upstream ENSMUSE00000699074 Chr5:34630748..34631086 CAGATTCCGAAAGTGGCTGT Chr5:34630759..34630778 60.26 50
upstream ENSMUSE00000699078 Chr5:34630748..34631098 CAGATTCCGAAAGTGGCTGT Chr5:34630759..34630778 60.26 50
upstream ENSMUSE00000716260 Chr5:34630748..34630975 CAGATTCCGAAAGTGGCTGT Chr5:34630759..34630778 60.26 50
upstream ENSMUSE00000242641 Chr5:34585822..34585962 CAGAGGTGCACACTGCTTGT Chr5:34585844..34585863 60.1 55
upstream ENSMUSE00000600726 Chr5:34585822..34586208 CAGAGGTGCACACTGCTTGT Chr5:34585844..34585863 60.1 55
upstream ENSMUSE00000242633 Chr5:34578652..34578807 GAGATCCGGCATGACAACTT Chr5:34578680..34578699 60.08 50
upstream ENSMUSE00000600725 Chr5:34578652..34578789 GAGATCCGGCATGACAACTT Chr5:34578680..34578699 60.08 50
upstream ENSMUSE00000242626 Chr5:34577061..34577136 No primer for this exon
upstream ENSMUSE00000600724 Chr5:34576934..34577136 GCACTCCGAGACCTCAACAC Chr5:34577000..34577019 60.87 60

*** Putative Vector Insertion (Chr 5: 34576044 - 34576933) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000600723 Chr5:34575954..34576043 GAACAGCACGATGACCTCCT Chr5:34575949..34575968 60.27 55
downstream ENSMUSE00000600722 Chr5:34574803..34574892 CCTGGGTCAGATACCCAAAA Chr5:34574842..34574861 59.78 50
downstream ENSMUSE00000699077 Chr5:34569237..34569540 GGCACAGACAGAAGGGTTTC Chr5:34569306..34569325 59.7 55
downstream ENSMUSE00000600721 Chr5:34567601..34567702 CAGCTCAGACATGTCCTCCA Chr5:34567614..34567633 59.98 55
downstream ENSMUSE00000699082 Chr5:34564618..34565106 TTTGCTTCTTCTGCCAGCTT Chr5:34564676..34564695 60.27 45
downstream ENSMUSE00000600720 Chr5:34559210..34560514 AGCATCCTCTGCACTCACCT Chr5:34559814..34559833 60.02 55
downstream ENSMUSE00000600719 Chr5:34542242..34542393 ACCTGGACCGGATCTTCTCT Chr5:34542268..34542287 60.07 55
downstream ENSMUSE00000242573 Chr5:34541428..34541544 CAGGTCGCTAGCATAGTTCG Chr5:34541482..34541501 58.7 55
downstream ENSMUSE00000242562 Chr5:34540131..34540235 ACTCCGCAGGGTCTGAGTTA Chr5:34540194..34540213 59.87 55
downstream ENSMUSE00000242552 Chr5:34539226..34539329 TGACGGTGAAGGGAGCTTTA Chr5:34539239..34539258 60.77 50
downstream ENSMUSE00000654189 Chr5:34537523..34538749 CAGAGCGTGTGCAATCCTTA Chr5:34537511..34537530 60.01 50
downstream ENSMUSE00000335208 Chr5:34537521..34538749 CAGAGCGTGTGCAATCCTTA Chr5:34537511..34537530 60.01 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCTTTTGCTTAATCGCCTTG Chr5:34576871..34576891 59.99 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector

Come back to gene ENSMUSG00000037224