Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI23748
Trapped Gene
Btk (ENSMUSG00000031264)
Vector Insertion
Chr X: 131107847 - 131108455
Public Clones not available
Private Clones OST246172 (lexicon) OST208854 (lexicon) OST173473 (lexicon)
Severity of mutation (?) Insertion after 10% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000712195 (ChrX:131108456..131108626 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CTTCAAGAAGCGCCTGTTTC ChrX:131108506..131108525 60.13 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000712195 (ChrX:131108456..131108626 -)
Downstram Exon
ENSMUSE00000207746 (ChrX:131107748..131107846 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CTTCAAGAAGCGCCTGTTTC ChrX:131108506..131108525 60.13 50 CTTTCTGGTGGGGGATTTTT ChrX:131107736..131107755 60.16 45

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000622602 ChrX:131117550..131117655 CTGGGGAGCTACCTGCATTA ChrX:131117563..131117582 60.23 55
upstream ENSMUSE00000694799 ChrX:131117550..131117667 CTGGGGAGCTACCTGCATTA ChrX:131117563..131117582 60.23 55
upstream ENSMUSE00000694798 ChrX:131112009..131112163 GGATTGCATCCTAAGGAAAGG ChrX:131112029..131112049 59.92 47.62
upstream ENSMUSE00000277353 ChrX:131108456..131108626 CTTCAAGAAGCGCCTGTTTC ChrX:131108506..131108525 60.13 50
upstream ENSMUSE00000712195 ChrX:131108456..131108626 CTTCAAGAAGCGCCTGTTTC ChrX:131108506..131108525 60.13 50

*** Putative Vector Insertion (Chr X: 131107847 - 131108455) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000207746 ChrX:131107748..131107846 CTTTCTGGTGGGGGATTTTT ChrX:131107736..131107755 60.16 45
downstream ENSMUSE00000207739 ChrX:131103444..131103512 AATGGGTACGGGAACCTTTC ChrX:131103426..131103445 60.05 50
downstream ENSMUSE00000207741 ChrX:131100136..131100217 GCTCTTCAGTTGGGGAGAAA ChrX:131100147..131100166 59.4 50
downstream ENSMUSE00000277330 ChrX:131093737..131093865 CAAAATTTGGCAGCCCATAG ChrX:131093731..131093750 60.45 45
downstream ENSMUSE00000277322 ChrX:131093226..131093293 GGGAAGAGGCTTTTTCGTTT ChrX:131093225..131093244 59.7 45
downstream ENSMUSE00000207752 ChrX:131091327..131091514 GACCTTTTTCAGCTCGGTTG ChrX:131091424..131091443 59.85 50
downstream ENSMUSE00000207745 ChrX:131090877..131090939 CTTGGGATGTAGCCTTCCTG ChrX:131090897..131090916 59.69 55
downstream ENSMUSE00000207740 ChrX:131090088..131090142 TGACTTCGAGTCATGTGCTTG ChrX:131090091..131090111 60.04 47.62
downstream ENSMUSE00000207736 ChrX:131089228..131089307 CCAGCTTTGCTGGAGTCTCT ChrX:131089242..131089261 59.74 55
downstream ENSMUSE00000694803 ChrX:131089002..131089065 GCGTGGAACACACAACGTAA ChrX:131089000..131089019 60.62 50
downstream ENSMUSE00000207748 ChrX:131088938..131089065 GCGTGGAACACACAACGTAA ChrX:131089000..131089019 60.62 50
downstream ENSMUSE00000207750 ChrX:131085741..131085815 TAGAAGGCGCGTTTTTGTTT ChrX:131085737..131085756 59.89 40
downstream ENSMUSE00000207751 ChrX:131084846..131085017 ATTCATCCTCCGACATGGAA ChrX:131084849..131084868 60.28 45
downstream ENSMUSE00000207743 ChrX:131083954..131084170 AAGGAACTGCTTCGACTCCA ChrX:131083944..131083963 59.99 50
downstream ENSMUSE00000207753 ChrX:131082101..131082165 No primer for this exon
downstream ENSMUSE00000207742 ChrX:131081251..131081369 TTGGAGCCTACAGAGCTGGT ChrX:131081306..131081325 60.01 55
downstream ENSMUSE00000207747 ChrX:131080021..131080178 ATGCCAGATGAGGCCTGTAG ChrX:131080040..131080059 60.24 55
downstream ENSMUSE00000694802 ChrX:131076980..131077346 GTATGCCAGGGAACGAGAGA ChrX:131077086..131077105 60.22 55
downstream ENSMUSE00000368789 ChrX:131076914..131077346 GTATGCCAGGGAACGAGAGA ChrX:131077086..131077105 60.22 55
downstream ENSMUSE00000694797 ChrX:131076909..131077346 GTATGCCAGGGAACGAGAGA ChrX:131077086..131077105 60.22 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TGGGGTAAGTTTCCCAAATG ChrX:131108438..131108458 59.66 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TGGGGTAAGTTTCCCAAATG ChrX:131108438..131108458 59.66 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000031264