Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI23798
Trapped Gene
Dbf4 (ENSMUSG00000002297)
Vector Insertion
Chr 5: 8410061 - 8412120
Public Clones IST14520D10 (tigm) IST10458G5 (tigm)
Private Clones OST244551 (lexicon) OST205733 (lexicon) OST100733 (lexicon) OST58553 (lexicon)
OST45371 (lexicon)
Severity of mutation (?) Insertion after 20% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000302266 (Chr5:8412121..8412300 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000302266 (Chr5:8412121..8412300 -)
Downstram Exon
ENSMUSE00000302255 (Chr5:8410010..8410060 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000496947 Chr5:8422467..8422680 No primer for this exon
upstream ENSMUSE00000497772 Chr5:8421221..8421393 No primer for this exon
upstream ENSMUSE00000302266 Chr5:8412121..8412300 No primer for this exon

*** Putative Vector Insertion (Chr 5: 8410061 - 8412120) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000302255 Chr5:8410010..8410060 No primer for this exon
downstream ENSMUSE00000302249 Chr5:8408494..8408563 No primer for this exon
downstream ENSMUSE00000184541 Chr5:8408234..8408310 No primer for this exon
downstream ENSMUSE00000184539 Chr5:8405802..8405838 No primer for this exon
downstream ENSMUSE00000184552 Chr5:8404871..8404916 No primer for this exon
downstream ENSMUSE00000184550 Chr5:8403612..8403740 No primer for this exon
downstream ENSMUSE00000184547 Chr5:8403080..8403191 No primer for this exon
downstream ENSMUSE00000184544 Chr5:8399688..8399812 No primer for this exon
downstream ENSMUSE00000346405 Chr5:8396973..8398162 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TTTGAGCCTAGGGGAAAGTG Chr5:8412080..8412100 59.31 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TTTGAGCCTAGGGGAAAGTG Chr5:8412080..8412100 59.31 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 TATAATCGCCTTGCAGCACAT Chr5:8412231..8412252 60.62 42.86 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 TCAAATACGTGACTGGGAAAAC Chr5:8412235..8412257 59 40.91 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000002297