Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI23891
Trapped Gene
Frrs1 (ENSMUSG00000033386)
Vector Insertion
Chr 3: 116583822 - 116584675
Public Clones not available
Private Clones OST241363 (lexicon) OST165500 (lexicon) OST95986 (lexicon)
Severity of mutation (?) Insertion after 19% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000323278 (Chr3:116583685..116583821 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CTTTTGGAAGCTCGTGATGC Chr3:116583717..116583736 60.91 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000323278 (Chr3:116583685..116583821 +)
Downstram Exon
ENSMUSE00000323273 (Chr3:116584676..116584770 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CTTTTGGAAGCTCGTGATGC Chr3:116583717..116583736 60.91 50 GCTTGGAGCATCCCAGTAGA Chr3:116584747..116584766 60.36 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000347028 Chr3:116581145..116581368 TCAGACGACGTTCAAACCTG Chr3:116581334..116581353 59.87 50
upstream ENSMUSE00000323278 Chr3:116583685..116583821 CTTTTGGAAGCTCGTGATGC Chr3:116583717..116583736 60.91 50

*** Putative Vector Insertion (Chr 3: 116583822 - 116584675) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000323273 Chr3:116584676..116584770 GCTTGGAGCATCCCAGTAGA Chr3:116584747..116584766 60.36 55
downstream ENSMUSE00000323268 Chr3:116585967..116586114 GGTTGGGAAATTACGGGACT Chr3:116586035..116586054 60.05 50
downstream ENSMUSE00000323260 Chr3:116588025..116588207 CTGGTCGTGAGAAAATGCAA Chr3:116588204..116588223 59.84 45
downstream ENSMUSE00000323252 Chr3:116593823..116593921 TCCTCACGGATGCACAAGTA Chr3:116593860..116593879 60.26 50
downstream ENSMUSE00000323242 Chr3:116594758..116594905 GGCCTTCTGCGAAGAATATG Chr3:116594896..116594915 59.81 50
downstream ENSMUSE00000323234 Chr3:116598406..116598519 CGGGACCCTCCTACACTCTT Chr3:116598496..116598515 60.5 60
downstream ENSMUSE00000323227 Chr3:116599596..116599711 ACCAGGACTCCAATGCTGAC Chr3:116599650..116599669 60.12 55
downstream ENSMUSE00000323222 Chr3:116602081..116602167 CGGTACACAAAGGGCAAAAC Chr3:116602154..116602173 60.4 50
downstream ENSMUSE00000323215 Chr3:116603522..116603619 TTGGGTCGTGTAAAGGTGGT Chr3:116603621..116603640 60.27 50
downstream ENSMUSE00000323207 Chr3:116603871..116603929 CCACACTCCAGTGAGTCCAG Chr3:116603908..116603927 59.27 60
downstream ENSMUSE00000323199 Chr3:116605232..116605375 CCCATCATTGCGTAGGTCTT Chr3:116605310..116605329 59.96 50
downstream ENSMUSE00000323188 Chr3:116605848..116605909 CTGCTTCTGCGACAGTCAGT Chr3:116605910..116605929 59.36 55
downstream ENSMUSE00000378090 Chr3:116605987..116606668 CTTCAGCGTTCCTCGATAGC Chr3:116606229..116606248 60.12 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CTCTGGGCTGCTTTTAATCG Chr3:116583859..116583879 59.97 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CTGCTTTCGTGACTGGGAAA Chr3:116583866..116583886 61.34 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000033386