Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI24007
Trapped Gene
Ppp1ca (ENSMUSG00000040385)
Vector Insertion
Chr 19: 4192486 - 4192744
Public Clones not available
Private Clones OST237946 (lexicon)
Severity of mutation (?) Insertion after 6% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000697240 (Chr19:4192434..4192485 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000697240 (Chr19:4192434..4192485 +)
Downstram Exon
ENSMUSE00000245885 (Chr19:4192745..4192876 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon ACAGACCACGGATCTCGTTC Chr19:4192807..4192826 60.12 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000245916 Chr19:4192178..4192271 GACAGCGAGAAGCTCAACCT Chr19:4192223..4192242 59.75 55
upstream ENSMUSE00000697240 Chr19:4192434..4192485 No primer for this exon

*** Putative Vector Insertion (Chr 19: 4192486 - 4192744) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000245885 Chr19:4192745..4192876 ACAGACCACGGATCTCGTTC Chr19:4192807..4192826 60.12 55
downstream ENSMUSE00000461425 Chr19:4193022..4193252 GCTTGCCCCGATCTACATAA Chr19:4193132..4193151 60.06 50
downstream ENSMUSE00000245838 Chr19:4193767..4193871 CAGGCAGTTGAAGCAGTCAG Chr19:4193828..4193847 59.77 55
downstream ENSMUSE00000245819 Chr19:4194467..4194690 AGGAGACACCACGGTCATTC Chr19:4194619..4194638 59.97 55
downstream ENSMUSE00000245799 Chr19:4194772..4194906 CTCCACAGTAGTTGGGAGCTG Chr19:4194849..4194869 59.92 57.14
downstream ENSMUSE00000394395 Chr19:4194982..4195419 CACAATCTGGTCTGCCATTG Chr19:4195340..4195359 60.11 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GAAGCTTAATCGCCTTGCAG Chr19:4192531..4192551 60.12 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AAGCTCGTGACTGGGAAAAC Chr19:4192532..4192552 59.33 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000040385