Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI24210
Trapped Gene
Mrpl23 (ENSMUSG00000037772)
Vector Insertion
Chr 7: 149719100 - 149720909
Public Clones CMHD-GT_507H11-3 (cmhd) IST14639H7 (tigm)
Private Clones OST230899 (lexicon) OST109867 (lexicon)
Severity of mutation (?) Insertion after 4% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000225470 (Chr7:149719080..149719099 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000225470 (Chr7:149719080..149719099 +)
Downstram Exon
ENSMUSE00000225458 (Chr7:149720910..149721032 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon GCACGGTATCTTCAGGCTGT Chr7:149721016..149721035 60.28 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000225470 Chr7:149719080..149719099 No primer for this exon

*** Putative Vector Insertion (Chr 7: 149719100 - 149720909) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000225458 Chr7:149720910..149721032 GCACGGTATCTTCAGGCTGT Chr7:149721016..149721035 60.28 55
downstream ENSMUSE00000225445 Chr7:149721975..149722057 AGCCACGGGAACATTGTAAA Chr7:149722035..149722054 60.37 45
downstream ENSMUSE00000225432 Chr7:149723172..149723245 TGCACGTAGGCCACTTTGTA Chr7:149723244..149723263 60.32 50
downstream ENSMUSE00000385249 Chr7:149726424..149726648 GTCATAGGCCAAACCAGGAA Chr7:149726571..149726590 59.93 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TAATCGCCTTGCAGCACATC Chr7:149719151..149719171 62.25 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector

Come back to gene ENSMUSG00000037772