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About
UniTrap UNI24250
Trapped Gene
Gtdc1 (
ENSMUSG00000036890
)
Vector Insertion
Chr 2: 44681064 - 44715810
Public Clones
IST14048E2 (tigm)
IST14252F2 (tigm)
IST14256F2 (tigm)
Private Clones
OST227014 (lexicon)
OST190424 (lexicon)
Severity of mutation
(?)
Insertion after 0% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000693250 (Chr2:44715811..44715846 -)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
No primer for this exon
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000693250 (Chr2:44715811..44715846 -)
Downstram Exon
ENSMUSE00000382626 (Chr2:44680871..44681063 -)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
No primer for this exon
GCTCTCCAGTGCCATTTCTT
Chr2:44680903..44680922
59.43
50
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000693251
Chr2:44717006..44717142
TCCTGACCTGCTTGAGTTCC
Chr2:44717045..44717064
60.39
55
upstream
ENSMUSE00000693250
Chr2:44715811..44715846
No primer for this exon
upstream
ENSMUSE00000708537
Chr2:44715811..44715855
No primer for this exon
upstream
ENSMUSE00000717993
Chr2:44715811..44715855
No primer for this exon
*** Putative Vector Insertion (Chr 2: 44681064 - 44715810) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000382626
Chr2:44680871..44681063
GCTCTCCAGTGCCATTTCTT
Chr2:44680903..44680922
59.43
50
downstream
ENSMUSE00000645480
Chr2:44680871..44681063
GCTCTCCAGTGCCATTTCTT
Chr2:44680903..44680922
59.43
50
downstream
ENSMUSE00000721982
Chr2:44680871..44681063
GCTCTCCAGTGCCATTTCTT
Chr2:44680903..44680922
59.43
50
downstream
ENSMUSE00000645476
Chr2:44644422..44644563
AGCAGCAGTTCCAGCTTCTC
Chr2:44644516..44644535
59.9
55
downstream
ENSMUSE00000275833
Chr2:44611788..44611955
GCAGCCAGTTCAGTCAGGTT
Chr2:44611889..44611908
60.45
55
downstream
ENSMUSE00000275828
Chr2:44607567..44607740
CCTTAGGCCTGTGATCAGGA
Chr2:44607615..44607634
60.21
55
downstream
ENSMUSE00000333997
Chr2:44490522..44490836
CATGTAATGTGCAGCGGTCT
Chr2:44490512..44490531
59.75
50
downstream
ENSMUSE00000712267
Chr2:44447401..44447510
No primer for this exon
downstream
ENSMUSE00000717339
Chr2:44447401..44447510
No primer for this exon
downstream
ENSMUSE00000275917
Chr2:44430963..44431107
TGCACAGTACGCGGAAATAG
Chr2:44430993..44431012
59.9
50
downstream
ENSMUSE00000693237
Chr2:44430963..44431107
TGCACAGTACGCGGAAATAG
Chr2:44430993..44431012
59.9
50
downstream
ENSMUSE00000275903
Chr2:44426733..44426803
GCTTTGGGACAGAGTGGGTA
Chr2:44426737..44426756
60.11
55
downstream
ENSMUSE00000693234
Chr2:44426733..44426803
GCTTTGGGACAGAGTGGGTA
Chr2:44426737..44426756
60.11
55
downstream
ENSMUSE00000275891
Chr2:44425939..44426030
TGGCCTCTTGCAGAAACTCT
Chr2:44425944..44425963
60.13
50
downstream
ENSMUSE00000693232
Chr2:44425939..44426030
TGGCCTCTTGCAGAAACTCT
Chr2:44425944..44425963
60.13
50
downstream
ENSMUSE00000645479
Chr2:44419942..44421054
GAATTCAGGCTCCGTGTCAT
Chr2:44420555..44420574
60.08
50
downstream
ENSMUSE00000693230
Chr2:44419942..44421054
GAATTCAGGCTCCGTGTCAT
Chr2:44420555..44420574
60.08
50
downstream
ENSMUSE00000693248
Chr2:44419932..44421054
GAATTCAGGCTCCGTGTCAT
Chr2:44420555..44420574
60.08
50
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
AACTGCAGGGTTCTGGAATTT
Chr2:44709811..44709832
59.99
42.86
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
ATGGAGATTTTGTTCACAGTTGAC
Chr2:44715828..44715852
59.44
37.5
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
PCR 3
TTCTAATCGCCTTGCAGCAC
Chr2:44703779..44703799
61.44
50
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 4
TAATGGCGTGACTGGGAAAA
Chr2:44703782..44703802
61.39
45
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000036890