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CBM UniTrap Project

Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI24652
Trapped Gene
CR956641.9 (ENSMUSG00000083534)
Vector Insertion
Chr 17: 37106263 - 37106384
Public Clones not available
Private Clones OST211186 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000719869 (Chr17:37106385..37106666 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
AGGTCATGGACCTTGTGGAG Chr17:37106511..37106530 59.96 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000719869 (Chr17:37106385..37106666 -)
Downstram Exon
ENSMUSE00000719236 (Chr17:37106140..37106262 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
AGGTCATGGACCTTGTGGAG Chr17:37106511..37106530 59.96 55 CCCCAAGGAGAACTACACCA Chr17:37106184..37106203 59.96 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000718339 Chr17:37108263..37108341 TCCAGACCAAGAACCAGAGC Chr17:37108264..37108283 60.39 55
upstream ENSMUSE00000711268 Chr17:37107786..37108058 GCAGTTTATGCGCTTTGACA Chr17:37107945..37107964 60.02 45
upstream ENSMUSE00000717541 Chr17:37107266..37107542 GGCAGAAGGTGTAGCAGAGG Chr17:37107351..37107370 60.01 60
upstream ENSMUSE00000719869 Chr17:37106385..37106666 AGGTCATGGACCTTGTGGAG Chr17:37106511..37106530 59.96 55

*** Putative Vector Insertion (Chr 17: 37106263 - 37106384) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000719236 Chr17:37106140..37106262 CCCCAAGGAGAACTACACCA Chr17:37106184..37106203 59.96 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CTCCTGAGTAATCGCCTTGC Chr17:37106321..37106341 59.98 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TCACCCTGAGATGGGGTAAG Chr17:37106378..37106398 59.92 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000083534