Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI25351
Trapped Gene
Athl1 (ENSMUSG00000062031)
Vector Insertion
Chr 7: 148129675 - 148130543
Public Clones not available
Private Clones OST187069 (lexicon)
Severity of mutation (?) Insertion after 68% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000587045 (Chr7:148129176..148129674 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GGCCACGTACTTAACCCTGA Chr7:148129186..148129205 59.99 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000587045 (Chr7:148129176..148129674 +)
Downstram Exon
ENSMUSE00000481117 (Chr7:148130544..148130663 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GGCCACGTACTTAACCCTGA Chr7:148129186..148129205 59.99 55 CAGCGTACGGACTCGGTATT Chr7:148130627..148130646 60.15 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000709516 Chr7:148127480..148127542 CCCACAGAGCCTTCTTACACC Chr7:148127491..148127511 61.05 57.14
upstream ENSMUSE00000720480 Chr7:148127480..148127542 CCCACAGAGCCTTCTTACACC Chr7:148127491..148127511 61.05 57.14
upstream ENSMUSE00000512462 Chr7:148128184..148128464 CGGTGTGTACAATGGAGCTG Chr7:148128337..148128356 60.17 55
upstream ENSMUSE00000587046 Chr7:148128184..148128464 CGGTGTGTACAATGGAGCTG Chr7:148128337..148128356 60.17 55
upstream ENSMUSE00000484192 Chr7:148128885..148129095 AGGGAACAAACCCATCACAG Chr7:148129000..148129019 59.82 50
upstream ENSMUSE00000486100 Chr7:148129176..148129611 GGCCACGTACTTAACCCTGA Chr7:148129186..148129205 59.99 55
upstream ENSMUSE00000587045 Chr7:148129176..148129674 GGCCACGTACTTAACCCTGA Chr7:148129186..148129205 59.99 55

*** Putative Vector Insertion (Chr 7: 148129675 - 148130543) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000481117 Chr7:148130544..148130663 CAGCGTACGGACTCGGTATT Chr7:148130627..148130646 60.15 55
downstream ENSMUSE00000482904 Chr7:148130850..148130981 TGGGTCCCATAAATGTCCTC Chr7:148130923..148130942 59.6 50
downstream ENSMUSE00000474028 Chr7:148131137..148131248 CCTGGGAGCTCCATTCTACA Chr7:148131230..148131249 60.21 55
downstream ENSMUSE00000475013 Chr7:148131354..148131426 TGGACCAGGACATTGGTGTA Chr7:148131423..148131442 59.81 50
downstream ENSMUSE00000476066 Chr7:148131525..148131661 GATCCTATCAGCCACCTCCA Chr7:148131606..148131625 60.03 55
downstream ENSMUSE00000668389 Chr7:148131529..148131661 GATCCTATCAGCCACCTCCA Chr7:148131606..148131625 60.03 55
downstream ENSMUSE00000470951 Chr7:148131779..148131912 TTTTTCCTTCGGATGTCAGG Chr7:148131863..148131882 60.04 45
downstream ENSMUSE00000472014 Chr7:148132006..148132101 AGTGACATTGGCGAAACTCC Chr7:148132092..148132111 60.12 50
downstream ENSMUSE00000499518 Chr7:148132230..148132327 CCCATGCCAGTCAAGAAGTT Chr7:148132288..148132307 60.11 50
downstream ENSMUSE00000500545 Chr7:148132401..148132612 GTCACCGAGTCCTTGGAAAA Chr7:148132526..148132545 60.09 50
downstream ENSMUSE00000587043 Chr7:148132420..148132620 GTCACCGAGTCCTTGGAAAA Chr7:148132526..148132545 60.09 50
downstream ENSMUSE00000501570 Chr7:148132688..148133557 AGCTAAGCCCCTGACTGTGA Chr7:148133148..148133167 60.01 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TTACACAGGGGGAGTTGCAT Chr7:148129634..148129654 60.38 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TTACACAGGGGGAGTTGCAT Chr7:148129634..148129654 60.38 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000062031