Tools for the biologist enabling optimized use of gene trap clones
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About
UniTrap UNI25402
Trapped Gene
1700061G19Rik (
ENSMUSG00000024209
)
Vector Insertion
Chr 17: 57015104 - 57015510
Public Clones
IST10376H2 (tigm)
IST12250A5 (tigm)
IST12066B3 (tigm)
IST12012E1 (tigm)
IST12250A5 (tigm)
IST12257H5 (tigm)
IST12066B3 (tigm)
IST10376H11 (tigm)
IST12526C1 (tigm)
IST12526C1BBF1 (tigm)
IST11852B1 (tigm)
IST12257H5 (tigm)
IST12012E1 (tigm)
IST10376H2 (tigm)
Private Clones
OST185345 (lexicon)
Severity of mutation
(?)
Insertion after 0% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000284015 (Chr17:57014906..57015103 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
TGATAAAGTGGCAGCCTGTG
Chr17:57015009..57015028
59.86
50
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000284015 (Chr17:57014906..57015103 +)
Downstram Exon
ENSMUSE00000283990 (Chr17:57015511..57015819 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
TGATAAAGTGGCAGCCTGTG
Chr17:57015009..57015028
59.86
50
GAGGACCAGGTGTCCACAGT
Chr17:57015632..57015651
60.01
60
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000284015
Chr17:57014906..57015103
TGATAAAGTGGCAGCCTGTG
Chr17:57015009..57015028
59.86
50
*** Putative Vector Insertion (Chr 17: 57015104 - 57015510) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000283990
Chr17:57015511..57015819
GAGGACCAGGTGTCCACAGT
Chr17:57015632..57015651
60.01
60
downstream
ENSMUSE00000283969
Chr17:57016786..57017012
TGGCCTTCTCGCTTAGATGT
Chr17:57016822..57016841
59.98
50
downstream
ENSMUSE00000376498
Chr17:57019874..57019962
ACCATGAAAGCGTTCCAGAC
Chr17:57019900..57019919
60.12
50
downstream
ENSMUSE00000283885
Chr17:57020369..57020489
AGGTCTCAGCGATGACTTGG
Chr17:57020436..57020455
60.41
55
downstream
ENSMUSE00000493939
Chr17:57021590..57021670
ATGCTTCAAGTAGCCCTGGA
Chr17:57021613..57021632
59.84
50
downstream
ENSMUSE00000283830
Chr17:57021964..57022113
TGATTGGGCTTCAGTGTGTC
Chr17:57022046..57022065
59.68
50
downstream
ENSMUSE00000139757
Chr17:57022203..57022409
GAAGCAAAGTGGCAGGTAGC
Chr17:57022301..57022320
60.02
55
downstream
ENSMUSE00000139751
Chr17:57022739..57022920
CAGCTTCTTGTTGGTGTGGA
Chr17:57022915..57022934
59.87
50
downstream
ENSMUSE00000283755
Chr17:57023050..57023283
ACTCCGACAAGCCGTACAAC
Chr17:57023237..57023256
60.18
55
downstream
ENSMUSE00000139749
Chr17:57024174..57024391
CCACCTTCCTCTCTGTGCTC
Chr17:57024313..57024332
59.99
60
downstream
ENSMUSE00000139761
Chr17:57024507..57024646
GGGATACGGGTTGACCATTT
Chr17:57024553..57024572
60.81
50
downstream
ENSMUSE00000283440
Chr17:57025864..57026110
AGGTTACTCCGAGCCTCTCC
Chr17:57025907..57025926
59.84
60
downstream
ENSMUSE00000693744
Chr17:57028060..57028327
GGCGCTTGAACGAAGATTAG
Chr17:57028182..57028201
59.98
50
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
GCCACTAGGCACAGAAGGTC
Chr17:57015088..57015108
59.87
60
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
GCCACTAGGCACAGAAGGTC
Chr17:57015088..57015108
59.87
60
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000024209