Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI25465
Trapped Gene
Igf2bp3 (ENSMUSG00000029814)
Vector Insertion
Chr 6: 49163327 - 49164295
Public Clones not available
Private Clones OST182888 (lexicon) OST149720 (lexicon) OST77003 (lexicon)
Severity of mutation (?) Insertion after 10% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000473559 (Chr6:49164296..49164956 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CATTCAAAAAGCCGGACACT Chr6:49164920..49164939 60.11 45 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000473559 (Chr6:49164296..49164956 -)
Downstram Exon
ENSMUSE00000292807 (Chr6:49163266..49163326 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CATTCAAAAAGCCGGACACT Chr6:49164920..49164939 60.11 45 TAGGGACCGAGTGCTCAACT Chr6:49163254..49163273 59.87 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000473559 Chr6:49164296..49164956 CATTCAAAAAGCCGGACACT Chr6:49164920..49164939 60.11 45

*** Putative Vector Insertion (Chr 6: 49163327 - 49164295) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000292807 Chr6:49163266..49163326 TAGGGACCGAGTGCTCAACT Chr6:49163254..49163273 59.87 55
downstream ENSMUSE00000292799 Chr6:49118469..49118517 GTAAGTGGGGCGGGATATTT Chr6:49118455..49118474 60.04 50
downstream ENSMUSE00000292793 Chr6:49084846..49084897 ACAGCTCTCCACCACTCCAT Chr6:49084831..49084850 59.71 55
downstream ENSMUSE00000292786 Chr6:49084694..49084757 TACAACTGCCGTTTCCGAAT Chr6:49084707..49084726 60.5 45
downstream ENSMUSE00000292782 Chr6:49067151..49067432 TTTGTGATGTTGCGAATGGT Chr6:49067144..49067163 59.97 40
downstream ENSMUSE00000193031 Chr6:49058925..49059059 AGAGGTGCCTTCAGGGGTAG Chr6:49058959..49058978 60.64 60
downstream ENSMUSE00000193028 Chr6:49057341..49057463 CCGTGTCTTGCTCGATTTTT Chr6:49057341..49057360 60.25 45
downstream ENSMUSE00000193030 Chr6:49055569..49055704 CTTGGCACACGTCTCAACAC Chr6:49055610..49055629 60.36 55
downstream ENSMUSE00000193029 Chr6:49054173..49054298 AACAGACCCAAGGCATTCAG Chr6:49054227..49054246 60.11 50
downstream ENSMUSE00000193026 Chr6:49044139..49044255 GCGGGAATAAACAGATGCAC Chr6:49044196..49044215 60.48 50
downstream ENSMUSE00000498311 Chr6:49040975..49041049 ATCACCATCCGCACTTTAGC Chr6:49040984..49041003 60.1 50
downstream ENSMUSE00000193027 Chr6:49038429..49038560 AAAGGACGGCACTCTGATGT Chr6:49038443..49038462 59.73 50
downstream ENSMUSE00000193033 Chr6:49037767..49037880 GGGACGACAACTTCAGCACT Chr6:49037815..49037834 60.31 55
downstream ENSMUSE00000388923 Chr6:49035227..49037439 GATCACCCGCACCTACAAGT Chr6:49035857..49035876 60 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ATCTAATCGCCTTGCAGCAC Chr6:49164227..49164247 60.38 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CCATCCGTGACTGGGAAAAC Chr6:49164229..49164249 62.63 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000029814