Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI25533
Trapped Gene
Got2 (ENSMUSG00000031672)
Vector Insertion
Chr 8: 98389152 - 98390720
Public Clones not available
Private Clones OST178597 (lexicon)
Severity of mutation (?) Insertion after 91% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000211654 (Chr8:98390721..98390871 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GTTCTGTTTCACCGGCCTAA Chr8:98390732..98390751 60.11 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000211654 (Chr8:98390721..98390871 -)
Downstram Exon
ENSMUSE00000417378 (Chr8:98388036..98389151 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GTTCTGTTTCACCGGCCTAA Chr8:98390732..98390751 60.11 50 TCTCTCCACGGCTCTTTGAT Chr8:98388301..98388320 59.95 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000387897 Chr8:98412117..98412301 CCAGATCTCGGCTCTACCAC Chr8:98412232..98412251 59.83 60
upstream ENSMUSE00000211651 Chr8:98401613..98401769 TACCGGGATGATAACGGAAA Chr8:98401641..98401660 60.15 45
upstream ENSMUSE00000211659 Chr8:98399646..98399774 CTGGGCGAGAACAATGAAGT Chr8:98399662..98399681 60.26 50
upstream ENSMUSE00000211652 Chr8:98398304..98398363 TTCGTCACTGTGCAGACCAT Chr8:98398344..98398363 60.32 50
upstream ENSMUSE00000211650 Chr8:98396076..98396237 ATGCAGCTCCAAGGTTATCG Chr8:98396134..98396153 60.24 50
upstream ENSMUSE00000240581 Chr8:98395791..98395895 AGATAGCGTCCGTGGTGAAG Chr8:98395791..98395810 60.28 55
upstream ENSMUSE00000211656 Chr8:98394675..98394825 CCAATCGTATGCCAAGAACA Chr8:98394687..98394706 59.54 45
upstream ENSMUSE00000211655 Chr8:98393372..98393537 TTGATCCGTCCCCTGTATTC Chr8:98393441..98393460 59.75 50
upstream ENSMUSE00000211654 Chr8:98390721..98390871 GTTCTGTTTCACCGGCCTAA Chr8:98390732..98390751 60.11 50

*** Putative Vector Insertion (Chr 8: 98389152 - 98390720) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000417378 Chr8:98388036..98389151 TCTCTCCACGGCTCTTTGAT Chr8:98388301..98388320 59.95 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CCCTTTGCAAGCCAGTAATC Chr8:98390664..98390684 59.71 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ACCAGATCGGCATGTTCTGT Chr8:98390743..98390763 60.54 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000031672