Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI25583
Trapped Gene
1810032O08Rik (ENSMUSG00000020812)
Vector Insertion
Chr 11: 116533071 - 116533477
Public Clones IST10311E11 (tigm) IST14136B6 (tigm)
Private Clones OST175320 (lexicon)
Severity of mutation (?) Insertion after 19% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000714124 (Chr11:116532995..116533070 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000714124 (Chr11:116532995..116533070 +)
Downstram Exon
ENSMUSE00000645978 (Chr11:116533478..116533669 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000669674 Chr11:116532983..116533070 No primer for this exon
upstream ENSMUSE00000712316 Chr11:116532995..116533070 No primer for this exon
upstream ENSMUSE00000714124 Chr11:116532995..116533070 No primer for this exon

*** Putative Vector Insertion (Chr 11: 116533071 - 116533477) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000645978 Chr11:116533478..116533669 No primer for this exon
downstream ENSMUSE00000717803 Chr11:116533478..116533669 No primer for this exon
downstream ENSMUSE00000109477 Chr11:116533978..116534052 No primer for this exon
downstream ENSMUSE00000386181 Chr11:116535252..116535385 No primer for this exon
downstream ENSMUSE00000669672 Chr11:116535614..116537112 No primer for this exon
downstream ENSMUSE00000669677 Chr11:116535614..116535689 No primer for this exon
downstream ENSMUSE00000669676 Chr11:116536822..116537110 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGGCCTAAGCTTTGGGTGAT Chr11:116533051..116533071 60.1 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGGCCTAAGCTTTGGGTGAT Chr11:116533051..116533071 60.1 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000020812