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About
UniTrap UNI25613
Trapped Gene
Bat3 (
ENSMUSG00000024392
)
Vector Insertion
Chr 17: 35275435 - 35275530
Public Clones
not available
Private Clones
OST173915 (lexicon)
Severity of mutation
(?)
Insertion after 7% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000607957 (Chr17:35275317..35275434 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
GAAACAGCGGCTCATCTACC
Chr17:35275373..35275392
59.84
55
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000607957 (Chr17:35275317..35275434 +)
Downstram Exon
ENSMUSE00000607956 (Chr17:35275531..35275727 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
GAAACAGCGGCTCATCTACC
Chr17:35275373..35275392
59.84
55
CATGAGTTGCTGAGGCAGAG
Chr17:35275635..35275654
59.73
55
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000480073
Chr17:35272187..35272416
AGTAAATCGGGGGAGACTGG
Chr17:35272303..35272322
60.32
55
upstream
ENSMUSE00000697507
Chr17:35272204..35272416
AGTAAATCGGGGGAGACTGG
Chr17:35272303..35272322
60.32
55
upstream
ENSMUSE00000345660
Chr17:35273060..35273180
AGCCGAGTGATAGTGCCAGT
Chr17:35273077..35273096
59.9
55
upstream
ENSMUSE00000607957
Chr17:35275317..35275434
GAAACAGCGGCTCATCTACC
Chr17:35275373..35275392
59.84
55
*** Putative Vector Insertion (Chr 17: 35275435 - 35275530) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000607956
Chr17:35275531..35275727
CATGAGTTGCTGAGGCAGAG
Chr17:35275635..35275654
59.73
55
downstream
ENSMUSE00000607955
Chr17:35275968..35276021
GTGAACATCCACAGCAGAGC
Chr17:35275997..35276016
59.42
55
downstream
ENSMUSE00000607954
Chr17:35276180..35276254
TCCGGGACAGTAAGGTCTGT
Chr17:35276252..35276271
59.57
55
downstream
ENSMUSE00000607953
Chr17:35277178..35277434
CAAGGCTACCGTCTCTGAGG
Chr17:35277261..35277280
60.01
60
downstream
ENSMUSE00000607952
Chr17:35277767..35277896
ACCTCGCAGTAGCGCTGTAG
Chr17:35277862..35277881
60.75
60
downstream
ENSMUSE00000607951
Chr17:35278371..35278568
CACGAAAGTGTTGCCCAGTA
Chr17:35278451..35278470
59.76
50
downstream
ENSMUSE00000607950
Chr17:35278775..35279027
TGGTCACAGTAGTCCCCACA
Chr17:35278802..35278821
60
55
downstream
ENSMUSE00000607949
Chr17:35279252..35279329
GGTCTGTCCATGACCAGGAG
Chr17:35279328..35279347
60.53
60
downstream
ENSMUSE00000607928
Chr17:35279443..35279550
GCCTGATGAGTGATCTGGTG
Chr17:35279521..35279540
59.21
55
downstream
ENSMUSE00000657453
Chr17:35279672..35279784
GAGTTGGTGCTGTTGGGAAG
Chr17:35279710..35279729
60.69
55
downstream
ENSMUSE00000474183
Chr17:35279868..35279955
TCTGGGCCAATGAAGTGTTT
Chr17:35279910..35279929
60.49
45
downstream
ENSMUSE00000607926
Chr17:35279871..35279955
TCTGGGCCAATGAAGTGTTT
Chr17:35279910..35279929
60.49
45
downstream
ENSMUSE00000657452
Chr17:35280278..35280627
AAATCAGTCATGCCCTGGAG
Chr17:35280623..35280642
60.07
50
downstream
ENSMUSE00000657451
Chr17:35281096..35281387
TCTCAGGACCTAAGCCTCCA
Chr17:35281228..35281247
59.94
55
downstream
ENSMUSE00000657450
Chr17:35281544..35281713
GAGAGCAGAGCTCCGAAGAA
Chr17:35281568..35281587
59.97
55
downstream
ENSMUSE00000657449
Chr17:35281891..35281944
CATACTCTTCCAGCCCAGTGA
Chr17:35281932..35281952
60.26
52.38
downstream
ENSMUSE00000657448
Chr17:35282082..35282184
TCCGGATGATATCCACACCT
Chr17:35282124..35282143
60.16
50
downstream
ENSMUSE00000657447
Chr17:35282274..35282398
CCATTGATGACAGCAGCAAG
Chr17:35282394..35282413
60.41
50
downstream
ENSMUSE00000657446
Chr17:35282551..35282697
AGGATCACCAACCCTACGAA
Chr17:35282694..35282713
59.4
50
downstream
ENSMUSE00000657445
Chr17:35282807..35282866
TGAACTTCCATCGGCTCTTC
Chr17:35282838..35282857
60.34
50
downstream
ENSMUSE00000657444
Chr17:35282971..35283114
CTGTTCATCTCGGGAACCTC
Chr17:35283069..35283088
59.65
55
downstream
ENSMUSE00000714083
Chr17:35283191..35283298
ATGCCACTGAGGTAGGCATC
Chr17:35283282..35283301
60.1
55
downstream
ENSMUSE00000722169
Chr17:35283191..35283298
ATGCCACTGAGGTAGGCATC
Chr17:35283282..35283301
60.1
55
downstream
ENSMUSE00000141843
Chr17:35283702..35283848
GCTCTCCTGAACCTCTGGTG
Chr17:35283839..35283858
59.99
60
downstream
ENSMUSE00000697491
Chr17:35283702..35283848
GCTCTCCTGAACCTCTGGTG
Chr17:35283839..35283858
59.99
60
downstream
ENSMUSE00000544727
Chr17:35284086..35284181
GGGGCTGTAGTTGGGATCTT
Chr17:35284139..35284158
60.33
55
downstream
ENSMUSE00000697492
Chr17:35284086..35284250
AAAGCTAGGGGTCATCAGCA
Chr17:35284188..35284207
59.84
50
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
AGCTTAAGGCTGCTGTCTGG
Chr17:35275456..35275476
59.78
55
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
AGCTTAAGGCTGCTGTCTGG
Chr17:35275456..35275476
59.78
55
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000024392