Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI25726
Trapped Gene
1700001P01Rik (ENSMUSG00000018543)
Vector Insertion
Chr 11: 97634130 - 97636965
Public Clones IST11377F7 (tigm) IST11377F7 (tigm) IST13139B11 (tigm) IST14315C3 (tigm)
IST13139B11 (tigm)
Private Clones OST168919 (lexicon) OST126370 (lexicon)
Severity of mutation (?) Insertion after 40% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000705938 (Chr11:97636966..97637191 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000705938 (Chr11:97636966..97637191 -)
Downstram Exon
ENSMUSE00000577583 (Chr11:97634030..97634129 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000382080 Chr11:97636966..97637232 No primer for this exon
upstream ENSMUSE00000705938 Chr11:97636966..97637191 No primer for this exon

*** Putative Vector Insertion (Chr 11: 97634130 - 97636965) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000577583 Chr11:97634030..97634129 No primer for this exon
downstream ENSMUSE00000577581 Chr11:97632897..97633051 No primer for this exon
downstream ENSMUSE00000521023 Chr11:97632842..97632895 No primer for this exon
downstream ENSMUSE00000673696 Chr11:97632795..97633051 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CGACTTCAGGCAGTCAGCTT Chr11:97636915..97636935 60.73 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ACTTCAGGCAGTCAGCTTGC Chr11:97636913..97636933 60.74 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 TTTCTTTTAATCGCCTTGCAG Chr11:97634127..97634148 59.5 38.1 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 GCTCAGTGAAGTCCCCATCT Chr11:97634215..97634235 59.26 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000018543