Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI25953
Trapped Gene
Mpped2 (ENSMUSG00000016386)
Vector Insertion
Chr 2: 106624016 - 106701644
Public Clones IST13985H9 (tigm)
Private Clones OST149371 (lexicon)
Severity of mutation (?) Insertion after 61% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000599133 (Chr2:106623790..106624015 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000599133 (Chr2:106623790..106624015 +)
Downstram Exon
ENSMUSE00000166268 (Chr2:106701645..106701760 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000686567 Chr2:106533426..106533978 No primer for this exon
upstream ENSMUSE00000686573 Chr2:106533426..106533971 No primer for this exon
upstream ENSMUSE00000642723 Chr2:106535758..106535833 No primer for this exon
upstream ENSMUSE00000686572 Chr2:106539441..106539692 No primer for this exon
upstream ENSMUSE00000405943 Chr2:106539445..106539692 No primer for this exon
upstream ENSMUSE00000599134 Chr2:106584857..106585038 No primer for this exon
upstream ENSMUSE00000599133 Chr2:106623790..106624015 No primer for this exon

*** Putative Vector Insertion (Chr 2: 106624016 - 106701644) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000166268 Chr2:106701645..106701760 No primer for this exon
downstream ENSMUSE00000166267 Chr2:106704812..106704925 No primer for this exon
downstream ENSMUSE00000642716 Chr2:106707105..106707359 No primer for this exon
downstream ENSMUSE00000686566 Chr2:106707105..106708513 No primer for this exon
downstream ENSMUSE00000686568 Chr2:106707105..106708510 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TTTAAAGGCCCAGGCTAACA Chr2:106699002..106699022 59.72 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GCCATACTGTTCGTGACTGG Chr2:106699056..106699076 59.17 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000016386