Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI26057
Trapped Gene
Cabp4 (ENSMUSG00000024842)
Vector Insertion
Chr 19: 4136016 - 4136113
Public Clones IST14570C2 (tigm) IST14570C2 (tigm)
Private Clones OST142536 (lexicon)
Severity of mutation (?) Insertion after 97% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000244392 (Chr19:4136114..4136261 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TGAGATGTTGCGAGAAATGG Chr19:4136147..4136166 59.8 45 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000244392 (Chr19:4136114..4136261 -)
Downstram Exon
ENSMUSE00000390567 (Chr19:4135425..4136015 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TGAGATGTTGCGAGAAATGG Chr19:4136147..4136166 59.8 45 GCCAGTAGGTTGTCCCTGAA Chr19:4135939..4135958 60.11 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000244492 Chr19:4139203..4139609 GCACTCCAGGGAGCTACATC Chr19:4139579..4139598 59.83 60
upstream ENSMUSE00000145468 Chr19:4138774..4138804 No primer for this exon
upstream ENSMUSE00000145460 Chr19:4138549..4138692 CTACCGCGAGCTAGGTGACT Chr19:4138621..4138640 59.66 60
upstream ENSMUSE00000434684 Chr19:4137107..4137216 AGCTGATAAGCCCAAAGCTG Chr19:4137164..4137183 59.62 50
upstream ENSMUSE00000244392 Chr19:4136114..4136261 TGAGATGTTGCGAGAAATGG Chr19:4136147..4136166 59.8 45

*** Putative Vector Insertion (Chr 19: 4136016 - 4136113) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000390567 Chr19:4135425..4136015 GCCAGTAGGTTGTCCCTGAA Chr19:4135939..4135958 60.11 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ATGCCTCTAGCACCGTGAAC Chr19:4136065..4136085 60.28 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ATGCCTCTAGCACCGTGAAC Chr19:4136065..4136085 60.28 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000024842