Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI26131
Trapped Gene
Nudt15 (ENSMUSG00000033405)
Vector Insertion
Chr 14: 73931993 - 73947840
Public Clones not available
Private Clones OST135436 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000378674 (Chr14:73947841..73948049 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TTCAGTGGGACCTGAAGACC Chr14:73947854..73947873 60.09 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000378674 (Chr14:73947841..73948049 -)
Downstram Exon
ENSMUSE00000364256 (Chr14:73931875..73931992 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TTCAGTGGGACCTGAAGACC Chr14:73947854..73947873 60.09 55 TTTCCAAACCCCAACATCTC Chr14:73931873..73931892 59.77 45

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000378674 Chr14:73947841..73948049 TTCAGTGGGACCTGAAGACC Chr14:73947854..73947873 60.09 55

*** Putative Vector Insertion (Chr 14: 73931993 - 73947840) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000364256 Chr14:73931875..73931992 TTTCCAAACCCCAACATCTC Chr14:73931873..73931892 59.77 45
downstream ENSMUSE00000411578 Chr14:73924867..73925123 ATTGCTCGCTGAGTGTTGTG Chr14:73925062..73925081 60.06 50
downstream ENSMUSE00000257555 Chr14:73923105..73923301 CTCTCTGAGCGCATTCTTCC Chr14:73923251..73923270 60.24 55
downstream ENSMUSE00000357022 Chr14:73919671..73921481 CTATGCTGTGCCTGACCAGA Chr14:73920375..73920394 60.01 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CCTTCCTCACTCTCGACCTG Chr14:73932790..73932810 59.98 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AAACCTTCGTGACTGGGAAA Chr14:73932776..73932796 59.57 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000033405