Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI26497
Trapped Gene
Paip2b (ENSMUSG00000045896)
Vector Insertion
Chr 6: 83760070 - 83764715
Public Clones not available
Private Clones OST114495 (lexicon) OST103571 (lexicon)
Severity of mutation (?) Insertion after 44% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000390940 (Chr6:83764716..83764863 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
ATGGATCCAGTGTGGCAAGT Chr6:83764830..83764849 60.4 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000390940 (Chr6:83764716..83764863 -)
Downstram Exon
ENSMUSE00000350403 (Chr6:83759893..83760069 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
ATGGATCCAGTGTGGCAAGT Chr6:83764830..83764849 60.4 50 GTCCTGGTCCTCTTCATCCA Chr6:83759973..83759992 60.05 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000653661 Chr6:83781629..83781735 AGGTACCTCGAGCGTTTGTG Chr6:83781705..83781724 60.31 55
upstream ENSMUSE00000559040 Chr6:83781265..83781322 GATGACGACGCTGAACACAG Chr6:83781275..83781294 60.47 55
upstream ENSMUSE00000390940 Chr6:83764716..83764863 ATGGATCCAGTGTGGCAAGT Chr6:83764830..83764849 60.4 50

*** Putative Vector Insertion (Chr 6: 83760070 - 83764715) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000350403 Chr6:83759893..83760069 GTCCTGGTCCTCTTCATCCA Chr6:83759973..83759992 60.05 55
downstream ENSMUSE00000615086 Chr6:83756056..83758886 CGGGTACAATTGTGCTTCCT Chr6:83756571..83756590 59.99 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TTGCAGAATACATGTGGATGG Chr6:83764740..83764761 59.41 42.86 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TTGCAGAATACATGTGGATGG Chr6:83764740..83764761 59.41 42.86 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000045896