Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI26637
Trapped Gene
Ube2m (ENSMUSG00000005575)
Vector Insertion
Chr 7: 13621822 - 13621914
Public Clones not available
Private Clones OST106398 (lexicon)
Severity of mutation (?) Insertion after 37% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000286070 (Chr7:13621915..13622009 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000286070 (Chr7:13621915..13622009 -)
Downstram Exon
ENSMUSE00000195441 (Chr7:13621783..13621821 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000365033 Chr7:13622970..13623327 No primer for this exon
upstream ENSMUSE00000286070 Chr7:13621915..13622009 No primer for this exon

*** Putative Vector Insertion (Chr 7: 13621822 - 13621914) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000195441 Chr7:13621783..13621821 No primer for this exon
downstream ENSMUSE00000195443 Chr7:13621561..13621664 No primer for this exon
downstream ENSMUSE00000481905 Chr7:13621191..13621254 No primer for this exon
downstream ENSMUSE00000408154 Chr7:13620588..13621103 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCAGGATTCCCAAGAACAGA Chr7:13621877..13621897 60.19 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GCAGGATTCCCAAGAACAGA Chr7:13621877..13621897 60.19 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000005575