Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI26877
Trapped Gene
Arl8a (ENSMUSG00000026426)
Vector Insertion
Chr 1: 137043678 - 137049047
Public Clones not available
Private Clones OST88770 (lexicon)
Severity of mutation (?) Insertion after 22% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000423940 (Chr1:137043411..137043677 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GACCATGATCGCTTTGTTCA Chr1:137043551..137043570 59.65 45 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000423940 (Chr1:137043411..137043677 +)
Downstram Exon
ENSMUSE00000241913 (Chr1:137049048..137049128 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GACCATGATCGCTTTGTTCA Chr1:137043551..137043570 59.65 45 TGGTGATTTTTCGCATGTTG Chr1:137049111..137049130 60.5 40

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000423940 Chr1:137043411..137043677 GACCATGATCGCTTTGTTCA Chr1:137043551..137043570 59.65 45

*** Putative Vector Insertion (Chr 1: 137043678 - 137049047) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000241913 Chr1:137049048..137049128 TGGTGATTTTTCGCATGTTG Chr1:137049111..137049130 60.5 40
downstream ENSMUSE00000241905 Chr1:137049370..137049443 AGTAGCGTTCCCACATGCTT Chr1:137049424..137049443 59.76 50
downstream ENSMUSE00000158963 Chr1:137050753..137050846 ATTCTTGGAGGCCTCGATCT Chr1:137050804..137050823 60.18 50
downstream ENSMUSE00000158967 Chr1:137050985..137051052 GCGAGGTCTCGCTTGTTACC Chr1:137051019..137051038 62.24 60
downstream ENSMUSE00000538969 Chr1:137051290..137051360 CAGATCTCTCGGTCCTGGAT Chr1:137051325..137051344 59.21 55
downstream ENSMUSE00000241867 Chr1:137051799..137052845 TACCTGGTGGCCCTATCAAG Chr1:137052489..137052508 59.95 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCAAGACCACCTTCGTCAAC Chr1:137046650..137046670 60.7 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GCAAGACCACCTTCGTCAAC Chr1:137046650..137046670 60.7 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000026426