Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI27076
Trapped Gene
Acp5 (ENSMUSG00000001348)
Vector Insertion
Chr 9: 21935099 - 21936180
Public Clones IST14632B3 (tigm)
Private Clones OST72860 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000702450 (Chr9:21936181..21936259 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000702450 (Chr9:21936181..21936259 -)
Downstram Exon
ENSMUSE00000702441 (Chr9:21934967..21935098 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000702450 Chr9:21936181..21936259 No primer for this exon

*** Putative Vector Insertion (Chr 9: 21935099 - 21936180) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000702441 Chr9:21934967..21935098 No primer for this exon
downstream ENSMUSE00000217906 Chr9:21934307..21934573 No primer for this exon
downstream ENSMUSE00000217900 Chr9:21933975..21934102 No primer for this exon
downstream ENSMUSE00000217902 Chr9:21932119..21932464 No primer for this exon
downstream ENSMUSE00000702440 Chr9:21931190..21931706 No primer for this exon
downstream ENSMUSE00000638189 Chr9:21931175..21931706 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCAGCTCAGTTGGGTAGCAC Chr9:21936204..21936224 61.01 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GCAGCTCAGTTGGGTAGCAC Chr9:21936204..21936224 61.01 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 CAGCTCAGTTGGGTTAATCG Chr9:21936203..21936223 58.38 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 AGCTCAGTTGGGTCGTGACT Chr9:21936202..21936222 59.91 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000001348