Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI27157
Trapped Gene
Bcl2l1 (ENSMUSG00000007659)
Vector Insertion
Chr 2: 152593196 - 152606407
Public Clones IST10954H5 (tigm) IST10954H5 (tigm) IST12818E7 (tigm) IST12742H10 (tigm)
IST12818E7 (tigm)
Private Clones OST68157 (lexicon)
Severity of mutation (?) Insertion after 80% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000556001 (Chr2:152606408..152608018 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000556001 (Chr2:152606408..152608018 -)
Downstram Exon
ENSMUSE00000556011 (Chr2:152593052..152593195 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000556013 Chr2:152657339..152657464 No primer for this exon
upstream ENSMUSE00000681713 Chr2:152656122..152656402 No primer for this exon
upstream ENSMUSE00000381992 Chr2:152655163..152655838 No primer for this exon
upstream ENSMUSE00000713045 Chr2:152655163..152655838 No primer for this exon
upstream ENSMUSE00000681711 Chr2:152655023..152655034 No primer for this exon
upstream ENSMUSE00000556001 Chr2:152606408..152608018 No primer for this exon
upstream ENSMUSE00000595156 Chr2:152606404..152608018 No primer for this exon

*** Putative Vector Insertion (Chr 2: 152593196 - 152606407) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000556011 Chr2:152593052..152593195 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGGGGGAAACTGCTGGTAAT Chr2:152603352..152603372 59.83 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGTTCCTCCGCCTTATCACC Chr2:152603355..152603375 60.46 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 TAATCGCCTTGCAGCACATC Chr2:152602029..152602049 62.25 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 GGGTCCCTCACACTATTTGG Chr2:152605005..152605025 59.26 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000007659