Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI27250
Trapped Gene
Slc10a7 (ENSMUSG00000031684)
Vector Insertion
Chr 8: 81033574 - 81039484
Public Clones (sanger) (sanger) E324D03 (ggtc) E325G04 (ggtc) E324D03 (ggtc)
E325G04 (ggtc) D131B03 (ggtc) IST12086A2 (tigm) IST10117E2 (tigm)
IST12947B2 (tigm)
Private Clones OST65758 (lexicon) OST57200 (lexicon) OST29589 (lexicon)
Severity of mutation (?) Insertion after 10% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000404952 (Chr8:81033267..81033573 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
AATGGTTCATGGTCGGGATA Chr8:81033502..81033521 60.01 45 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000404952 (Chr8:81033267..81033573 +)
Downstram Exon
ENSMUSE00000211734 (Chr8:81039485..81039567 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
AATGGTTCATGGTCGGGATA Chr8:81033502..81033521 60.01 45 GACGGCAATGTAGGACACAG Chr8:81039528..81039547 59.17 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000404952 Chr8:81033267..81033573 AATGGTTCATGGTCGGGATA Chr8:81033502..81033521 60.01 45
upstream ENSMUSE00000445432 Chr8:81033361..81033573 AATGGTTCATGGTCGGGATA Chr8:81033502..81033521 60.01 45

*** Putative Vector Insertion (Chr 8: 81033574 - 81039484) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000211734 Chr8:81039485..81039567 GACGGCAATGTAGGACACAG Chr8:81039528..81039547 59.17 55
downstream ENSMUSE00000581693 Chr8:81049061..81049197 GGGAAGAAGGCAAGTGTGAA Chr8:81049134..81049153 60.23 50
downstream ENSMUSE00000581696 Chr8:81049061..81049197 GGGAAGAAGGCAAGTGTGAA Chr8:81049134..81049153 60.23 50
downstream ENSMUSE00000249095 Chr8:81054982..81055057 GCATGCAACCTACTGTCTGC Chr8:81055007..81055026 59.48 55
downstream ENSMUSE00000581692 Chr8:81054982..81055057 GCATGCAACCTACTGTCTGC Chr8:81055007..81055026 59.48 55
downstream ENSMUSE00000249088 Chr8:81102618..81102656 No primer for this exon
downstream ENSMUSE00000606946 Chr8:81102618..81102656 No primer for this exon
downstream ENSMUSE00000249083 Chr8:81186886..81186921 AGCACCGGAGTCACAACAAT Chr8:81186911..81186930 60.58 50
downstream ENSMUSE00000606945 Chr8:81186886..81186921 AGCACCGGAGTCACAACAAT Chr8:81186911..81186930 60.58 50
downstream ENSMUSE00000606944 Chr8:81210484..81210567 GGAACCACCACCGTCATAAA Chr8:81210554..81210573 60.62 50
downstream ENSMUSE00000635949 Chr8:81210484..81210567 GGAACCACCACCGTCATAAA Chr8:81210554..81210573 60.62 50
downstream ENSMUSE00000211723 Chr8:81221065..81221230 GCTACTGCTGACCACACCAA Chr8:81221136..81221155 59.9 55
downstream ENSMUSE00000606943 Chr8:81221065..81221230 GCTACTGCTGACCACACCAA Chr8:81221136..81221155 59.9 55
downstream ENSMUSE00000211729 Chr8:81222419..81222470 AAGCTCAGCTGAACGGAGAC Chr8:81222443..81222462 59.75 55
downstream ENSMUSE00000682022 Chr8:81222419..81222470 AAGCTCAGCTGAACGGAGAC Chr8:81222443..81222462 59.75 55
downstream ENSMUSE00000211727 Chr8:81230774..81230847 TGGTGTGAACCCCGAGTTAT Chr8:81230798..81230817 60.23 50
downstream ENSMUSE00000682021 Chr8:81230774..81230847 TGGTGTGAACCCCGAGTTAT Chr8:81230798..81230817 60.23 50
downstream ENSMUSE00000211728 Chr8:81253517..81253662 CTCATGGCCTGCAAACACTA Chr8:81253557..81253576 59.86 50
downstream ENSMUSE00000606942 Chr8:81253517..81253662 CTCATGGCCTGCAAACACTA Chr8:81253557..81253576 59.86 50
downstream ENSMUSE00000341040 Chr8:81255508..81256050 CTGCACAAAGGCAGTACGAA Chr8:81255848..81255867 60.05 50
downstream ENSMUSE00000581694 Chr8:81255508..81257902 GAGGTACCTGACGCTTCTCG Chr8:81256058..81256077 60.01 60

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TTCTGAATAATCGCCTTGCAG Chr8:81036618..81036639 60.35 42.86 L232 GATGTGCTGCAAGGCGATTA Gene trap vector

Come back to gene ENSMUSG00000031684