Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI27332
Trapped Gene
Fundc2 (ENSMUSG00000031198)
Vector Insertion
Chr X: 72632592 - 72636122
Public Clones not available
Private Clones OST63131 (lexicon)
Severity of mutation (?) Insertion after 38% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000362065 (ChrX:72632441..72632591 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TGAAGCACTGGATCTTGCAG ChrX:72632460..72632479 60.14 50 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000362065 (ChrX:72632441..72632591 +)
Downstram Exon
ENSMUSE00000465895 (ChrX:72636123..72636198 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TGAAGCACTGGATCTTGCAG ChrX:72632460..72632479 60.14 50 AGCTGTTGCAGCCAATTTTC ChrX:72636174..72636193 60.4 45

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000425823 ChrX:72627768..72627790 AATGGCCGCTAACAGTCAAG ChrX:72627771..72627790 60.27 50
upstream ENSMUSE00000362065 ChrX:72632441..72632591 TGAAGCACTGGATCTTGCAG ChrX:72632460..72632479 60.14 50

*** Putative Vector Insertion (Chr X: 72632592 - 72636122) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000465895 ChrX:72636123..72636198 AGCTGTTGCAGCCAATTTTC ChrX:72636174..72636193 60.4 45
downstream ENSMUSE00000207194 ChrX:72636979..72637110 CCTCAGCTTTGCTCTTGACC ChrX:72637111..72637130 60.13 55
downstream ENSMUSE00000353252 ChrX:72639649..72640087 AAGACCTTGCACCAGGAGAA ChrX:72639897..72639916 59.84 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGGGGTGTTACTGGATGGTA ChrX:72632576..72632596 59.53 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GGGGGTGTTACTGGATGGTA ChrX:72632576..72632596 59.53 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000031198