Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI2739
Trapped Gene
Cenpf (ENSMUSG00000026605)
Vector Insertion
Chr 1: 191466682 - 191470680
Public Clones AK0408 (sanger) XP0023 (sanger) AW0634 (sanger)
Private Clones not available
Severity of mutation (?) Insertion after 98% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000160940 (Chr1:191470681..191470859 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GGTTTGCTGACATCCCAACT Chr1:191470840..191470859 59.97 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000160940 (Chr1:191470681..191470859 -)
Downstram Exon
ENSMUSE00000390024 (Chr1:191464495..191466681 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GGTTTGCTGACATCCCAACT Chr1:191470840..191470859 59.97 50 TTCCAACAGTGAAACCACCA Chr1:191465346..191465365 59.98 45

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000432810 Chr1:191511855..191511965 GAGCCAGGTTCTGTGAGGAG Chr1:191511860..191511879 59.99 60
upstream ENSMUSE00000336714 Chr1:191508824..191509017 AGCTTGAAGGACAGCTGGAG Chr1:191508902..191508921 59.74 55
upstream ENSMUSE00000374126 Chr1:191507647..191507843 CTGAGCTCATGCAAAAAGCA Chr1:191507677..191507696 60.28 45
upstream ENSMUSE00000333698 Chr1:191506709..191506830 AGAAGCCAACAAGTTGCACA Chr1:191506792..191506811 59.49 45
upstream ENSMUSE00000412799 Chr1:191506240..191506331 No primer for this exon
upstream ENSMUSE00000372309 Chr1:191504100..191504385 GCTCTGAAAACCCCACTGAG Chr1:191504246..191504265 59.84 55
upstream ENSMUSE00000410181 Chr1:191502818..191503020 GCTGGAGAAGACGAAAGTGG Chr1:191502904..191502923 59.99 55
upstream ENSMUSE00000341784 Chr1:191496388..191496513 ACCGACAGAACGCAGAGAGT Chr1:191496442..191496461 60.06 55
upstream ENSMUSE00000389189 Chr1:191494927..191495055 GTCCCGACAGCATCAATCTT Chr1:191495031..191495050 60.08 50
upstream ENSMUSE00000398942 Chr1:191492395..191492517 AAACGAGCTGAGGAGAAGCA Chr1:191492400..191492419 60.28 50
upstream ENSMUSE00000360638 Chr1:191485876..191486011 TCAGTCTGAGCAGAGGACCA Chr1:191485960..191485979 59.69 55
upstream ENSMUSE00000432761 Chr1:191483190..191483951 GGGATTTGCTCAAGTCCAGA Chr1:191483227..191483246 60.19 50
upstream ENSMUSE00000160935 Chr1:191480524..191483147 TCAAGCAAAGAGGTCCGAGT Chr1:191481299..191481318 59.99 50
upstream ENSMUSE00000289743 Chr1:191476140..191478971 ACGGTGCTCTTCCTTGAGAA Chr1:191477249..191477268 59.99 50
upstream ENSMUSE00000160936 Chr1:191475027..191475179 CAACACAGCTGAGCGAAGAC Chr1:191475096..191475115 59.78 55
upstream ENSMUSE00000160937 Chr1:191474441..191474600 GAAGGAACTTCAGCGTGCTC Chr1:191474549..191474568 60.14 55
upstream ENSMUSE00000160938 Chr1:191472490..191472966 GAAGTTGCTCGGCTGAAGTC Chr1:191472783..191472802 60.14 55
upstream ENSMUSE00000160940 Chr1:191470681..191470859 GGTTTGCTGACATCCCAACT Chr1:191470840..191470859 59.97 50

*** Putative Vector Insertion (Chr 1: 191466682 - 191470680) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000390024 Chr1:191464495..191466681 TTCCAACAGTGAAACCACCA Chr1:191465346..191465365 59.98 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGTAATCGCCTTGCAGCACA Chr1:191470611..191470631 61.9 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TTTACAGCGTGACTGGGAAA Chr1:191470616..191470636 59.32 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000026605