Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI27681
Trapped Gene
Sap30l (ENSMUSG00000020519)
Vector Insertion
Chr 11: 57615792 - 57619545
Public Clones not available
Private Clones OST53700 (lexicon) OST53699 (lexicon)
Severity of mutation (?) Insertion after 36% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000362685 (Chr11:57615139..57615791 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000362685 (Chr11:57615139..57615791 +)
Downstram Exon
ENSMUSE00000105597 (Chr11:57619546..57619668 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000362685 Chr11:57615139..57615791 No primer for this exon

*** Putative Vector Insertion (Chr 11: 57615792 - 57619545) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000105597 Chr11:57619546..57619668 No primer for this exon
downstream ENSMUSE00000105598 Chr11:57621526..57621624 No primer for this exon
downstream ENSMUSE00000335155 Chr11:57623444..57623716 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CTAATCGCCTTGCAGCACAT Chr11:57618842..57618862 61.32 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CACCAATCCAACCACAAGAA Chr11:57618773..57618793 59.39 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000020519