Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI27871
Trapped Gene
Evi5 (ENSMUSG00000011831)
Vector Insertion
Chr 5: 108271320 - 108304007
Public Clones (sanger) IST14240F2 (tigm) IST14629D12 (tigm) IST14330B3 (tigm) IST13153F11 (tigm)
IST11537C6 (tigm) IST14761G1 (tigm) IST13201H7 (tigm) IST14275C4 (tigm)
IST12895F6 (tigm)
Private Clones OST45415 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000719086 (Chr5:108304008..108304126 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000719086 (Chr5:108304008..108304126 -)
Downstram Exon
ENSMUSE00000651435 (Chr5:108271090..108271319 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000716444 Chr5:108304008..108304126 No primer for this exon
upstream ENSMUSE00000719086 Chr5:108304008..108304126 No primer for this exon
upstream ENSMUSE00000721175 Chr5:108304008..108304126 No primer for this exon

*** Putative Vector Insertion (Chr 5: 108271320 - 108304007) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000541284 Chr5:108271090..108271319 No primer for this exon
downstream ENSMUSE00000651435 Chr5:108271090..108271319 No primer for this exon
downstream ENSMUSE00000254281 Chr5:108250724..108250913 No primer for this exon
downstream ENSMUSE00000459398 Chr5:108249451..108249675 No primer for this exon
downstream ENSMUSE00000459394 Chr5:108248674..108248697 No primer for this exon
downstream ENSMUSE00000459390 Chr5:108248631..108248669 No primer for this exon
downstream ENSMUSE00000651434 Chr5:108248623..108248628 No primer for this exon
downstream ENSMUSE00000651440 Chr5:108248623..108248697 No primer for this exon
downstream ENSMUSE00000596113 Chr5:108247908..108248033 No primer for this exon
downstream ENSMUSE00000459379 Chr5:108245793..108245936 No primer for this exon
downstream ENSMUSE00000459374 Chr5:108244821..108244910 No primer for this exon
downstream ENSMUSE00000459367 Chr5:108244630..108244727 No primer for this exon
downstream ENSMUSE00000459363 Chr5:108242565..108242625 No primer for this exon
downstream ENSMUSE00000459359 Chr5:108241377..108241430 No primer for this exon
downstream ENSMUSE00000651438 Chr5:108239309..108239341 No primer for this exon
downstream ENSMUSE00000651437 Chr5:108238609..108238755 No primer for this exon
downstream ENSMUSE00000187588 Chr5:108228145..108228279 No primer for this exon
downstream ENSMUSE00000187578 Chr5:108226832..108226972 No primer for this exon
downstream ENSMUSE00000710399 Chr5:108224718..108224876 No primer for this exon
downstream ENSMUSE00000711532 Chr5:108224718..108224876 No primer for this exon
downstream ENSMUSE00000303496 Chr5:108219381..108219527 No primer for this exon
downstream ENSMUSE00000651432 Chr5:108219381..108219527 No primer for this exon
downstream ENSMUSE00000303490 Chr5:108217218..108217313 No primer for this exon
downstream ENSMUSE00000651431 Chr5:108217218..108217313 No primer for this exon
downstream ENSMUSE00000303483 Chr5:108193695..108193790 No primer for this exon
downstream ENSMUSE00000651430 Chr5:108193695..108193790 No primer for this exon
downstream ENSMUSE00000466933 Chr5:108173814..108177476 No primer for this exon
downstream ENSMUSE00000651429 Chr5:108173814..108177476 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ATAATCGCCTTGCAGCACAT Chr5:108279937..108279957 60.64 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CTGCTGCCTTTAGAAACCCTAA Chr5:108280016..108280038 59.91 45.46 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 TCGAACCCATTATGCCGTAG Chr5:108280112..108280132 60.85 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 TCGAACCCATTATGCCGTAG Chr5:108280112..108280132 60.85 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000011831