Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI28049
Trapped Gene
Creld1 (ENSMUSG00000030284)
Vector Insertion
Chr 6: 113441731 - 113441887
Public Clones not available
Private Clones OST41405 (lexicon)
Severity of mutation (?) Insertion after 65% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000197063 (Chr6:113441647..113441730 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TGGTACAGAGCAAGCCACCT Chr6:113441660..113441679 60.85 55 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000197063 (Chr6:113441647..113441730 +)
Downstram Exon
ENSMUSE00000197052 (Chr6:113441888..113441983 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TGGTACAGAGCAAGCCACCT Chr6:113441660..113441679 60.85 55 No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000224405 Chr6:113433554..113434070 GCACTGGTGGACAACTTCAA Chr6:113434047..113434066 59.73 50
upstream ENSMUSE00000197059 Chr6:113434489..113434571 AGAACCATCCGGGACAACTT Chr6:113434498..113434517 60.75 50
upstream ENSMUSE00000197055 Chr6:113438065..113438175 CTGGTGGAAAACTGGTGGTT Chr6:113438150..113438169 59.86 50
upstream ENSMUSE00000197051 Chr6:113438473..113438564 TCTGTTCCGATTCCCTGAAG Chr6:113438505..113438524 60.19 50
upstream ENSMUSE00000197061 Chr6:113439428..113439604 GTGGCCTTGGCTACTTTGAG Chr6:113439551..113439570 59.88 55
upstream ENSMUSE00000197065 Chr6:113439689..113439784 GAATCCCATTGTCTGCAGTG Chr6:113439727..113439746 59.09 50
upstream ENSMUSE00000197063 Chr6:113441647..113441730 TGGTACAGAGCAAGCCACCT Chr6:113441660..113441679 60.85 55

*** Putative Vector Insertion (Chr 6: 113441731 - 113441887) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000197052 Chr6:113441888..113441983 No primer for this exon
downstream ENSMUSE00000197050 Chr6:113442118..113442252 CCCTCCGTGTTTTCACACTT Chr6:113442184..113442203 60.01 50
downstream ENSMUSE00000377202 Chr6:113442681..113443333 TTTCATCCTCCGTCATCTCC Chr6:113442722..113442741 60.01 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TGGAGCTGACCAGTTCTGTG Chr6:113441682..113441702 60.02 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TGGAGCTGACCAGTTCTGTG Chr6:113441682..113441702 60.02 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000030284