Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI28190
Trapped Gene
Pcolce (ENSMUSG00000029718)
Vector Insertion
Chr 5: 138047029 - 138048054
Public Clones not available
Private Clones OST37642 (lexicon)
Severity of mutation (?) Insertion after 71% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000192039 (Chr5:138048055..138048335 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CGCTCTGACCCTAAGACAGG Chr5:138048153..138048172 60.01 60 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000192039 (Chr5:138048055..138048335 -)
Downstram Exon
ENSMUSE00000192036 (Chr5:138046951..138047028 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CGCTCTGACCCTAAGACAGG Chr5:138048153..138048172 60.01 60 CCTGACCGCTTGTACTGCTT Chr5:138046964..138046983 60.45 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000514567 Chr5:138052465..138052604 CAGACCCCCAACTACACGAG Chr5:138052465..138052484 60.56 60
upstream ENSMUSE00000648646 Chr5:138052465..138052632 CAGACCCCCAACTACACGAG Chr5:138052465..138052484 60.56 60
upstream ENSMUSE00000192035 Chr5:138051625..138051733 AGGTTACGTGGCAAGTGAGG Chr5:138051675..138051694 60.17 55
upstream ENSMUSE00000192034 Chr5:138050119..138050377 GCTACGATGCTCTGGAGGTC Chr5:138050291..138050310 59.98 60
upstream ENSMUSE00000686275 Chr5:138049845..138049919 No primer for this exon
upstream ENSMUSE00000192041 Chr5:138049183..138049307 CTGTTCCTGGCACATCATTG Chr5:138049197..138049216 60.11 50
upstream ENSMUSE00000192038 Chr5:138048636..138048772 TAACGGAGCTGTGAGTGACG Chr5:138048679..138048698 60.05 55
upstream ENSMUSE00000192039 Chr5:138048055..138048335 CGCTCTGACCCTAAGACAGG Chr5:138048153..138048172 60.01 60

*** Putative Vector Insertion (Chr 5: 138047029 - 138048054) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000192036 Chr5:138046951..138047028 CCTGACCGCTTGTACTGCTT Chr5:138046964..138046983 60.45 55
downstream ENSMUSE00000192037 Chr5:138046685..138046855 GCAGGGCACATACAACTTCA Chr5:138046688..138046707 59.72 50
downstream ENSMUSE00000590805 Chr5:138046336..138046545 CCTAGGTTGGGAGGGACACT Chr5:138046381..138046400 60.36 60
downstream ENSMUSE00000345501 Chr5:138046335..138046545 CCTAGGTTGGGAGGGACACT Chr5:138046381..138046400 60.36 60

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGTGGCCCCAGGTAAGTGTG Chr5:138048044..138048064 62.3 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGTGGCCCCAGGTAAGTGTG Chr5:138048044..138048064 62.3 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000029718