Tools for the biologist enabling optimized use of gene trap clones
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About
UniTrap UNI28198
Trapped Gene
Slc25a44 (
ENSMUSG00000050144
)
Vector Insertion
Chr 3: 88225061 - 88228649
Public Clones
not available
Private Clones
OST37469 (lexicon)
Severity of mutation
(?)
Insertion after 0% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000712761 (Chr3:88228650..88229061 -)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
CTGAGAAGGAAAGCGACCAC
Chr3:88228895..88228914
59.99
55
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000712761 (Chr3:88228650..88229061 -)
Downstram Exon
ENSMUSE00000673519 (Chr3:88224423..88225060 -)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
CTGAGAAGGAAAGCGACCAC
Chr3:88228895..88228914
59.99
55
CTGGATGTTCCGTTTGTCCT
Chr3:88225002..88225021
59.97
50
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000712761
Chr3:88228650..88229061
CTGAGAAGGAAAGCGACCAC
Chr3:88228895..88228914
59.99
55
upstream
ENSMUSE00000720948
Chr3:88228650..88229061
CTGAGAAGGAAAGCGACCAC
Chr3:88228895..88228914
59.99
55
*** Putative Vector Insertion (Chr 3: 88225061 - 88228649) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000360770
Chr3:88224423..88225060
CTGGATGTTCCGTTTGTCCT
Chr3:88225002..88225021
59.97
50
downstream
ENSMUSE00000673519
Chr3:88224423..88225060
CTGGATGTTCCGTTTGTCCT
Chr3:88225002..88225021
59.97
50
downstream
ENSMUSE00000673518
Chr3:88223964..88223973
No primer for this exon
downstream
ENSMUSE00000673517
Chr3:88223759..88223771
No primer for this exon
downstream
ENSMUSE00000673516
Chr3:88222291..88222304
No primer for this exon
downstream
ENSMUSE00000673515
Chr3:88220867..88220871
No primer for this exon
downstream
ENSMUSE00000363585
Chr3:88219910..88220037
GACAATGTGAGGGCACTCCT
Chr3:88219972..88219991
60.12
55
downstream
ENSMUSE00000673514
Chr3:88219908..88220011
ACATCCATGGGATTGGTGAG
Chr3:88219907..88219926
60.6
50
downstream
ENSMUSE00000403490
Chr3:88214421..88216819
GCAGAGCTGTCCAATCACAA
Chr3:88214876..88214895
59.99
50
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
AGATAATCGCCTTGCAGCAC
Chr3:88225581..88225601
60.38
50
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
CAGACGTGACTGGGAAAACC
Chr3:88225582..88225602
60.54
55
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000050144
