Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI28415
Trapped Gene
1700028P14Rik (ENSMUSG00000033053)
Vector Insertion
Chr 19: 23647033 - 23666598
Public Clones not available
Private Clones OST31531 (lexicon) OST31499 (lexicon) OST28420 (lexicon)
Severity of mutation (?) Insertion after 65% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000249837 (Chr19:23666599..23666667 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GGCTTTCGGGATGTTCCTAT Chr19:23666626..23666645 60.29 50 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000249837 (Chr19:23666599..23666667 -)
Downstram Exon
ENSMUSE00000249814 (Chr19:23646975..23647032 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GGCTTTCGGGATGTTCCTAT Chr19:23666626..23666645 60.29 50 No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000249934 Chr19:23727065..23727304 GCGGTGGTATGAGATTGGAC Chr19:23727150..23727169 60.35 55
upstream ENSMUSE00000249907 Chr19:23691009..23691114 TCTGGAGACGGTGAAAAACC Chr19:23691050..23691069 60.09 50
upstream ENSMUSE00000249857 Chr19:23667517..23667635 GTGAACAGATGGCCCAAGTT Chr19:23667597..23667616 59.97 50
upstream ENSMUSE00000249837 Chr19:23666599..23666667 GGCTTTCGGGATGTTCCTAT Chr19:23666626..23666645 60.29 50

*** Putative Vector Insertion (Chr 19: 23647033 - 23666598) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000249814 Chr19:23646975..23647032 No primer for this exon
downstream ENSMUSE00000249783 Chr19:23633250..23633527 CCATGTGTTGATGCCAAGTC Chr19:23633407..23633426 59.97 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TGGTTCACATCATTGCGTTC Chr19:23657595..23657615 60.53 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TATGAGCGTGACTGGGAAAAC Chr19:23657532..23657553 60.12 47.62 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000033053