Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI28812
Trapped Gene
Letm2 (ENSMUSG00000037363)
Vector Insertion
Chr 8: 26689277 - 26690191
Public Clones not available
Private Clones OST19354 (lexicon) OST12693 (lexicon)
Severity of mutation (?) Insertion after 96% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000637648 (Chr8:26690192..26690266 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CCCCAGTACCGTCATCACTC Chr8:26690225..26690244 60.39 60 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000637648 (Chr8:26690192..26690266 -)
Downstram Exon
ENSMUSE00000637647 (Chr8:26688967..26689276 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CCCCAGTACCGTCATCACTC Chr8:26690225..26690244 60.39 60 CCTTCAAGGGATCCTTAGGC Chr8:26689178..26689197 60.03 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000499277 Chr8:26707592..26707959 CTCCCCTACGGTGTACCTGA Chr8:26707860..26707879 59.98 60
upstream ENSMUSE00000523241 Chr8:26706834..26706917 No primer for this exon
upstream ENSMUSE00000406641 Chr8:26704191..26704638 AAGAAAAGCGGTCGTTGAGA Chr8:26704323..26704342 59.99 45
upstream ENSMUSE00000373214 Chr8:26702924..26703067 CTTCCGCTTGGTTCCATTTA Chr8:26703025..26703044 60.07 45
upstream ENSMUSE00000637654 Chr8:26697768..26697905 GCTGCCAAACTGGAAATAGC Chr8:26697862..26697881 59.85 50
upstream ENSMUSE00000637653 Chr8:26697079..26697279 CCAGCTCCTGATGACACTGA Chr8:26697102..26697121 59.98 55
upstream ENSMUSE00000637652 Chr8:26695821..26695940 AAGGGGTGAAGGCTTTGAGT Chr8:26695908..26695927 60.11 50
upstream ENSMUSE00000637650 Chr8:26692145..26692258 CCTCCACTTGAAGGAGAACG Chr8:26692231..26692250 59.84 55
upstream ENSMUSE00000637649 Chr8:26690788..26690880 CCTTGGAATACCGACACCTC Chr8:26690847..26690866 59.4 55
upstream ENSMUSE00000637648 Chr8:26690192..26690266 CCCCAGTACCGTCATCACTC Chr8:26690225..26690244 60.39 60

*** Putative Vector Insertion (Chr 8: 26689277 - 26690191) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000637647 Chr8:26688967..26689276 CCTTCAAGGGATCCTTAGGC Chr8:26689178..26689197 60.03 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ACTCATAGCACCAGCAGCAA Chr8:26690207..26690227 59.62 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ACTCATAGCACCAGCAGCAA Chr8:26690207..26690227 59.62 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000037363