Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI28841
Trapped Gene
Cap2 (ENSMUSG00000021373)
Vector Insertion
Chr 13: 46710725 - 46730989
Public Clones not available
Private Clones OST18233 (lexicon)
Severity of mutation (?) Insertion after 28% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000117401 (Chr13:46710619..46710724 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000117401 (Chr13:46710619..46710724 +)
Downstram Exon
ENSMUSE00000117405 (Chr13:46730990..46731179 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000718498 Chr13:46597494..46597580 No primer for this exon
upstream ENSMUSE00000571069 Chr13:46597516..46597580 No primer for this exon
upstream ENSMUSE00000278926 Chr13:46620784..46620905 No primer for this exon
upstream ENSMUSE00000720412 Chr13:46620784..46620905 No primer for this exon
upstream ENSMUSE00000278920 Chr13:46626346..46626446 No primer for this exon
upstream ENSMUSE00000490873 Chr13:46655873..46655950 No primer for this exon
upstream ENSMUSE00000278905 Chr13:46704965..46705108 No primer for this exon
upstream ENSMUSE00000117408 Chr13:46705433..46705518 No primer for this exon
upstream ENSMUSE00000117401 Chr13:46710619..46710724 No primer for this exon

*** Putative Vector Insertion (Chr 13: 46710725 - 46730989) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000117405 Chr13:46730990..46731179 No primer for this exon
downstream ENSMUSE00000117406 Chr13:46733218..46733390 No primer for this exon
downstream ENSMUSE00000117404 Chr13:46735192..46735315 No primer for this exon
downstream ENSMUSE00000571068 Chr13:46735409..46735491 No primer for this exon
downstream ENSMUSE00000117402 Chr13:46741895..46742035 No primer for this exon
downstream ENSMUSE00000278846 Chr13:46743650..46745634 No primer for this exon
downstream ENSMUSE00000719471 Chr13:46743650..46745004 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TCTTAATCGCCTTGCAGCAC Chr13:46719773..46719793 61.44 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGAGGCTAGGGTCGTGACTG Chr13:46719764..46719784 59.47 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000021373