Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI28851
Trapped Gene
1600014K23Rik (ENSMUSG00000031708)
Vector Insertion
Chr 8: 86097223 - 86097309
Public Clones not available
Private Clones OST17894 (lexicon)
Severity of mutation (?) Insertion after 18% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000212210 (Chr8:86097310..86097354 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000212210 (Chr8:86097310..86097354 -)
Downstram Exon
ENSMUSE00000212212 (Chr8:86097119..86097222 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon GGTCCCGGAAGTAGAGTGTG Chr8:86097123..86097142 59.57 60

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000681734 Chr8:86118239..86118361 ACTCAGTTGCAGCAGAGCAG Chr8:86118275..86118294 59.52 55
upstream ENSMUSE00000277901 Chr8:86098377..86098427 AAGACGAGGGAGAAGCTGTG Chr8:86098390..86098409 59.6 55
upstream ENSMUSE00000212217 Chr8:86097770..86097821 No primer for this exon
upstream ENSMUSE00000212210 Chr8:86097310..86097354 No primer for this exon

*** Putative Vector Insertion (Chr 8: 86097223 - 86097309) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000212212 Chr8:86097119..86097222 GGTCCCGGAAGTAGAGTGTG Chr8:86097123..86097142 59.57 60
downstream ENSMUSE00000212208 Chr8:86096927..86097042 ATTTGCGGCCATAAATGAAG Chr8:86096939..86096958 59.93 40
downstream ENSMUSE00000212216 Chr8:86096740..86096845 ATGGTTCCGTGAGAGAATCG Chr8:86096740..86096759 60.07 50
downstream ENSMUSE00000212209 Chr8:86096580..86096652 AGCCCCAATAGTAGGTGCAG Chr8:86096609..86096628 59.22 55
downstream ENSMUSE00000212213 Chr8:86096448..86096491 AGTGCCAGCTTAACCTGCTG Chr8:86096439..86096458 60.59 55
downstream ENSMUSE00000212207 Chr8:86096297..86096354 TGGATGGAGAAGTTCCCAAG Chr8:86096304..86096323 60.04 50
downstream ENSMUSE00000212215 Chr8:86096131..86096219 ACAGGAACAGCCAGGTGAAG Chr8:86096138..86096157 60.3 55
downstream ENSMUSE00000212206 Chr8:86095912..86095957 CTGAGTCAAGATGGCAAAGC Chr8:86095900..86095919 58.6 50
downstream ENSMUSE00000388157 Chr8:86095599..86095834 GTAGTCGCGGAACTCCTTCA Chr8:86095724..86095743 60.4 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GTCAGGAATAATCGCCTTGC Chr8:86097246..86097266 59.67 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGGAACGTGACTGGGAAAAC Chr8:86097243..86097263 59.04 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000031708