Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI29009
Trapped Gene
Mrps18c (ENSMUSG00000016833)
Vector Insertion
Chr 5: 101232130 - 101232988
Public Clones not available
Private Clones OST13061 (lexicon)
Severity of mutation (?) Insertion after 77% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000187937 (Chr5:101232072..101232129 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000187937 (Chr5:101232072..101232129 +)
Downstram Exon
ENSMUSE00000693553 (Chr5:101232989..101233048 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000693554 Chr5:101227756..101227879 No primer for this exon
upstream ENSMUSE00000693565 Chr5:101227762..101227879 No primer for this exon
upstream ENSMUSE00000406709 Chr5:101227766..101227879 No primer for this exon
upstream ENSMUSE00000187942 Chr5:101228571..101228632 No primer for this exon
upstream ENSMUSE00000187941 Chr5:101230927..101231010 No primer for this exon
upstream ENSMUSE00000187937 Chr5:101232072..101232129 No primer for this exon

*** Putative Vector Insertion (Chr 5: 101232130 - 101232988) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000187936 Chr5:101232989..101233048 No primer for this exon
downstream ENSMUSE00000693553 Chr5:101232989..101233048 No primer for this exon
downstream ENSMUSE00000693555 Chr5:101232989..101233490 No primer for this exon
downstream ENSMUSE00000353655 Chr5:101233351..101233477 No primer for this exon
downstream ENSMUSE00000693551 Chr5:101233351..101233480 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ATGCATTTATGGAAGGCACA Chr5:101232105..101232125 58.99 40 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ATGCATTTATGGAAGGCACA Chr5:101232105..101232125 58.99 40 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000016833