Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI29218
Trapped Gene
P42pop (ENSMUSG00000048481)
Vector Insertion
Chr 7: 19577970 - 19585839
Public Clones not available
Private Clones OST5401 (lexicon)
Severity of mutation (?) Insertion after 43% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000331959 (Chr7:19577331..19577969 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
AGGCTTCGACCGCTAGTACA Chr7:19577904..19577923 60.04 55 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000331959 (Chr7:19577331..19577969 +)
Downstram Exon
ENSMUSE00000442224 (Chr7:19585840..19586657 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
AGGCTTCGACCGCTAGTACA Chr7:19577904..19577923 60.04 55 TGGGATAGGCAAAGAGCATC Chr7:19586603..19586622 60.18 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000375506 Chr7:19576668..19576703 No primer for this exon
upstream ENSMUSE00000331959 Chr7:19577331..19577969 AGGCTTCGACCGCTAGTACA Chr7:19577904..19577923 60.04 55

*** Putative Vector Insertion (Chr 7: 19577970 - 19585839) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000442224 Chr7:19585840..19586657 TGGGATAGGCAAAGAGCATC Chr7:19586603..19586622 60.18 50
downstream ENSMUSE00000334311 Chr7:19586732..19587112 AGGCAAAGGACCCAACCTAT Chr7:19587017..19587036 59.83 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGGTGAGTCCCAAAGCCAGT Chr7:19577969..19577989 61.08 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TGAGTCCCAAAGCCAGTTTC Chr7:19577972..19577992 60.23 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000048481