Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI29282
Trapped Gene
Cnn1 (ENSMUSG00000001349)
Vector Insertion
Chr 9: 21903863 - 21905584
Public Clones (cmhd)
Private Clones OST828 (lexicon)
Severity of mutation (?) Insertion after 7% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000360963 (Chr9:21903725..21903862 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000360963 (Chr9:21903725..21903862 +)
Downstram Exon
ENSMUSE00000217875 (Chr9:21905585..21905706 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000360963 Chr9:21903725..21903862 No primer for this exon

*** Putative Vector Insertion (Chr 9: 21903863 - 21905584) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000217875 Chr9:21905585..21905706 No primer for this exon
downstream ENSMUSE00000217881 Chr9:21908750..21908816 No primer for this exon
downstream ENSMUSE00000442028 Chr9:21909814..21909951 No primer for this exon
downstream ENSMUSE00000217879 Chr9:21910114..21910224 No primer for this exon
downstream ENSMUSE00000538121 Chr9:21912195..21912341 No primer for this exon
downstream ENSMUSE00000217883 Chr9:21912430..21914074 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
  No PCR available

Come back to gene ENSMUSG00000001349