Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI29361
Trapped Gene
Ehbp1 (ENSMUSG00000042302)
Vector Insertion
Chr 11: 22148031 - 22185490
Public Clones Ayu21-T3 (egtc)
Private Clones not available
Severity of mutation (?) Insertion after 3% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000349890 (Chr11:22185491..22185841 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CTTGACTGGGCCATTGATCT Chr11:22185761..22185780 60.07 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000349890 (Chr11:22185491..22185841 -)
Downstram Exon
ENSMUSE00000272480 (Chr11:22147973..22148030 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CTTGACTGGGCCATTGATCT Chr11:22185761..22185780 60.07 50 TCTTGTCCAAACAACCACCA Chr11:22147975..22147994 59.98 45

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000349890 Chr11:22185491..22185841 CTTGACTGGGCCATTGATCT Chr11:22185761..22185780 60.07 50
upstream ENSMUSE00000719725 Chr11:22185491..22185841 CTTGACTGGGCCATTGATCT Chr11:22185761..22185780 60.07 50

*** Putative Vector Insertion (Chr 11: 22148031 - 22185490) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000272480 Chr11:22147973..22148030 TCTTGTCCAAACAACCACCA Chr11:22147975..22147994 59.98 45
downstream ENSMUSE00000272471 Chr11:22139146..22139241 GTTTTCAGGAACAGGCCAAA Chr11:22139151..22139170 60.09 45
downstream ENSMUSE00000272460 Chr11:22132005..22132058 No primer for this exon
downstream ENSMUSE00000272453 Chr11:22072828..22073009 AGAGTGGCAGACACGACCTT Chr11:22072851..22072870 59.91 55
downstream ENSMUSE00000272447 Chr11:22069038..22069177 TGTAATTTTTGCCGCCTTCT Chr11:22069017..22069036 59.72 40
downstream ENSMUSE00000272443 Chr11:22051791..22051895 TGATTTAGCTGAGGGCACATT Chr11:22051785..22051805 59.72 42.86
downstream ENSMUSE00000272438 Chr11:22051070..22051192 GCCAAGTCCTTTTGTGCTTC Chr11:22051122..22051141 59.86 50
downstream ENSMUSE00000680761 Chr11:22046537..22046703 TGGTTCATCAAATGGGTTCA Chr11:22046566..22046585 59.75 40
downstream ENSMUSE00000580848 Chr11:22046466..22046703 TGGTTCATCAAATGGGTTCA Chr11:22046566..22046585 59.75 40
downstream ENSMUSE00000580858 Chr11:22046466..22046628 TGGTTCATCAAATGGGTTCA Chr11:22046566..22046585 59.75 40
downstream ENSMUSE00000580857 Chr11:22037841..22038060 TCCCTGCAGAGACAACTGTG Chr11:22037842..22037861 60.02 55
downstream ENSMUSE00000272424 Chr11:22001055..22001233 CAGCGACTGACTAGCGTTTG Chr11:22001155..22001174 59.81 55
downstream ENSMUSE00000272416 Chr11:22000425..22000473 No primer for this exon
downstream ENSMUSE00000272409 Chr11:21995321..21996202 GGGCTCCGTGTCACTCTTAG Chr11:21995740..21995759 59.87 60
downstream ENSMUSE00000272398 Chr11:21989528..21989671 CCACCTTGAAAGCAGCATCT Chr11:21989562..21989581 60.4 50
downstream ENSMUSE00000272392 Chr11:21968362..21968509 TAGCTGACGAGCCCTCTCTC Chr11:21968437..21968456 59.85 60
downstream ENSMUSE00000272383 Chr11:21962783..21962890 AAGCCTTCAGGAATCTCTTCG Chr11:21962817..21962837 59.97 47.62
downstream ENSMUSE00000272373 Chr11:21959140..21959264 GCCTCGGTTACAGCTTTCTG Chr11:21959137..21959156 60.01 55
downstream ENSMUSE00000272366 Chr11:21956786..21956924 No primer for this exon
downstream ENSMUSE00000272357 Chr11:21956533..21956630 TCTGCCTTCTCCTCAGTTCC Chr11:21956548..21956567 59.53 55
downstream ENSMUSE00000272350 Chr11:21953435..21953558 TGCCAATTCCCCTACAACAT Chr11:21953492..21953511 60.19 45
downstream ENSMUSE00000410802 Chr11:21914858..21914957 AGGAGGGAAAGCTGGTTCAT Chr11:21914836..21914855 60.07 50
downstream ENSMUSE00000272337 Chr11:21913487..21913560 TTCAGCAGCTCATACCTTCG Chr11:21913497..21913516 59.17 50
downstream ENSMUSE00000272333 Chr11:21907086..21907200 GTTCATCCAGCAGGAGCTGT Chr11:21907125..21907144 60.42 55
downstream ENSMUSE00000373769 Chr11:21905831..21906874 TCCCAGGTCACCTTTCAAAC Chr11:21906120..21906139 59.94 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGTGTCAGCCTCCAGGAATA Chr11:22170475..22170495 60.07 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TAATTGCAGTCAGGCAGTCGT Chr11:22170436..22170457 60.84 47.62 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000042302