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About
UniTrap UNI29524
Trapped Gene
Cltc (
ENSMUSG00000047126
)
Vector Insertion
Chr 11: 86550572 - 86570995
Public Clones
(sanger)
P107F12 (ggtc)
IST10874G4 (tigm)
Private Clones
not available
Severity of mutation
(?)
Insertion after 1% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000661529 (Chr11:86570750..86570994 -)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
GAGACCATACTCCCCGACAG
Chr11:86570796..86570815
59.53
60
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000661529 (Chr11:86570750..86570994 -)
Downstram Exon
ENSMUSE00000675211 (Chr11:86550573..86550780 -)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
GAGACCATACTCCCCGACAG
Chr11:86570796..86570815
59.53
60
CAGGATTCATGATGGCACTG
Chr11:86550575..86550594
60.07
50
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000439974
Chr11:86570750..86570995
GAGACCATACTCCCCGACAG
Chr11:86570796..86570815
59.53
60
upstream
ENSMUSE00000661529
Chr11:86570750..86570994
GAGACCATACTCCCCGACAG
Chr11:86570796..86570815
59.53
60
upstream
ENSMUSE00000661528
Chr11:86550573..86550780
ATCCTGCCAGCAAAGTGATT
Chr11:86550583..86550602
59.7
45
upstream
ENSMUSE00000675211
Chr11:86550573..86550780
ATCCTGCCAGCAAAGTGATT
Chr11:86550583..86550602
59.7
45
*** Putative Vector Insertion (Chr 11: 86550572 - 86570995) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000366296
Chr11:86550561..86550780
CAGGATTCATGATGGCACTG
Chr11:86550575..86550594
60.07
50
downstream
ENSMUSE00000577864
Chr11:86547078..86547346
CAGTCATGGTATGGGCCTTC
Chr11:86547259..86547278
60.34
55
downstream
ENSMUSE00000675209
Chr11:86547078..86547346
CAGTCATGGTATGGGCCTTC
Chr11:86547259..86547278
60.34
55
downstream
ENSMUSE00000577863
Chr11:86546007..86546168
AGCTGCATGCCCTTCAATAG
Chr11:86546069..86546088
60.37
50
downstream
ENSMUSE00000675207
Chr11:86546007..86546168
AGCTGCATGCCCTTCAATAG
Chr11:86546069..86546088
60.37
50
downstream
ENSMUSE00000577862
Chr11:86544220..86544333
TGCATTGCAACAGGAAAGTC
Chr11:86544199..86544218
59.85
45
downstream
ENSMUSE00000675203
Chr11:86544220..86544333
TGCATTGCAACAGGAAAGTC
Chr11:86544199..86544218
59.85
45
downstream
ENSMUSE00000508302
Chr11:86543713..86543886
GATGGTTTCTCCGCTGATTC
Chr11:86543751..86543770
59.63
50
downstream
ENSMUSE00000675200
Chr11:86543713..86543886
GATGGTTTCTCCGCTGATTC
Chr11:86543751..86543770
59.63
50
downstream
ENSMUSE00000391484
Chr11:86539757..86539954
AAATTTCCGGGCAAAGAGTT
Chr11:86539798..86539817
59.94
40
downstream
ENSMUSE00000675198
Chr11:86539757..86539954
AAATTTCCGGGCAAAGAGTT
Chr11:86539798..86539817
59.94
40
downstream
ENSMUSE00000350882
Chr11:86538573..86538773
CGACGGATGGTATCTGGAGT
Chr11:86538720..86538739
59.95
55
downstream
ENSMUSE00000675196
Chr11:86538573..86538773
CGACGGATGGTATCTGGAGT
Chr11:86538720..86538739
59.95
55
downstream
ENSMUSE00000373263
Chr11:86535677..86535829
GGTACATTGGCCCTGAGGTA
Chr11:86535722..86535741
59.81
55
downstream
ENSMUSE00000675193
Chr11:86535677..86535829
GGTACATTGGCCCTGAGGTA
Chr11:86535722..86535741
59.81
55
downstream
ENSMUSE00000407509
Chr11:86535077..86535199
TGATCCGCATGACATTTCTC
Chr11:86535126..86535145
59.61
45
downstream
ENSMUSE00000675191
Chr11:86535077..86535199
TGATCCGCATGACATTTCTC
Chr11:86535126..86535145
59.61
45
downstream
ENSMUSE00000387053
Chr11:86533752..86533889
GAGGTGCGTGCATAAGGTTC
Chr11:86533732..86533751
60.67
55
downstream
ENSMUSE00000675189
Chr11:86533752..86533889
GAGGTGCGTGCATAAGGTTC
Chr11:86533732..86533751
60.67
55
downstream
ENSMUSE00000348745
Chr11:86532961..86533125
No primer for this exon
downstream
ENSMUSE00000675186
Chr11:86532961..86533125
No primer for this exon
downstream
ENSMUSE00000389072
Chr11:86532688..86532868
TAGCTGACAGCATGGCTCTG
Chr11:86532774..86532793
60.31
55
downstream
ENSMUSE00000675184
Chr11:86532688..86532868
TAGCTGACAGCATGGCTCTG
Chr11:86532774..86532793
60.31
55
downstream
ENSMUSE00000336457
Chr11:86531400..86531563
GCTCGGGATCATAGCAGTTG
Chr11:86531398..86531417
60.76
55
downstream
ENSMUSE00000675183
Chr11:86531400..86531563
GCTCGGGATCATAGCAGTTG
Chr11:86531398..86531417
60.76
55
downstream
ENSMUSE00000362532
Chr11:86526083..86526208
CCAGGTCATGGACAAAGTCA
Chr11:86526117..86526136
59.52
50
downstream
ENSMUSE00000675180
Chr11:86526083..86526208
CCAGGTCATGGACAAAGTCA
Chr11:86526117..86526136
59.52
50
downstream
ENSMUSE00000350824
Chr11:86525349..86525491
GAGCAGTCAACATCCAGCAA
Chr11:86525414..86525433
59.99
50
downstream
ENSMUSE00000675178
Chr11:86525349..86525491
GAGCAGTCAACATCCAGCAA
Chr11:86525414..86525433
59.99
50
downstream
ENSMUSE00000392135
Chr11:86524843..86525077
CACACTGGCCACGTTCATAA
Chr11:86524838..86524857
60.57
50
downstream
ENSMUSE00000675175
Chr11:86524843..86525077
CACACTGGCCACGTTCATAA
Chr11:86524838..86524857
60.57
50
downstream
ENSMUSE00000333361
Chr11:86522452..86522574
TGGATCCTTTCGACGGACTA
Chr11:86522490..86522509
60.59
50
downstream
ENSMUSE00000675174
Chr11:86522452..86522574
TGGATCCTTTCGACGGACTA
Chr11:86522490..86522509
60.59
50
downstream
ENSMUSE00000395972
Chr11:86520972..86521117
TGGGTCCTGAGTTTCAGACA
Chr11:86521060..86521079
59.23
50
downstream
ENSMUSE00000675172
Chr11:86520972..86521117
TGGGTCCTGAGTTTCAGACA
Chr11:86521060..86521079
59.23
50
downstream
ENSMUSE00000345377
Chr11:86520554..86520737
CGTGTTCGGTCAGCCTTAAT
Chr11:86520665..86520684
60.13
50
downstream
ENSMUSE00000675171
Chr11:86520554..86520737
CGTGTTCGGTCAGCCTTAAT
Chr11:86520665..86520684
60.13
50
downstream
ENSMUSE00000389324
Chr11:86520169..86520361
AGCCTGGACAACTTCCATGT
Chr11:86520160..86520179
59.58
50
downstream
ENSMUSE00000675167
Chr11:86520169..86520361
AGCCTGGACAACTTCCATGT
Chr11:86520160..86520179
59.58
50
downstream
ENSMUSE00000348198
Chr11:86519475..86519632
CTAATTCTGCAAGGCGGTTT
Chr11:86519494..86519513
59.36
45
downstream
ENSMUSE00000649333
Chr11:86519475..86519632
CTAATTCTGCAAGGCGGTTT
Chr11:86519494..86519513
59.36
45
downstream
ENSMUSE00000412568
Chr11:86518648..86518812
CAACTGCTGCCTGATACTCG
Chr11:86518667..86518686
59.62
55
downstream
ENSMUSE00000649332
Chr11:86518648..86518812
CAACTGCTGCCTGATACTCG
Chr11:86518667..86518686
59.62
55
downstream
ENSMUSE00000362493
Chr11:86518390..86518497
TGGAGTCCACACATCTGAGC
Chr11:86518417..86518436
59.83
55
downstream
ENSMUSE00000649331
Chr11:86518390..86518497
TGGAGTCCACACATCTGAGC
Chr11:86518417..86518436
59.83
55
downstream
ENSMUSE00000383813
Chr11:86518142..86518309
TCTCGACCAGAACAACTCCA
Chr11:86518135..86518154
59.39
50
downstream
ENSMUSE00000649330
Chr11:86518142..86518309
TCTCGACCAGAACAACTCCA
Chr11:86518135..86518154
59.39
50
downstream
ENSMUSE00000343542
Chr11:86517582..86517731
TGTCTTTGAACTGCCCTTCC
Chr11:86517571..86517590
60.23
50
downstream
ENSMUSE00000649329
Chr11:86517582..86517731
TGTCTTTGAACTGCCCTTCC
Chr11:86517571..86517590
60.23
50
downstream
ENSMUSE00000407896
Chr11:86517373..86517504
ACTGCACGAGTGTGATCCAA
Chr11:86517367..86517386
60.32
50
downstream
ENSMUSE00000649328
Chr11:86517373..86517504
ACTGCACGAGTGTGATCCAA
Chr11:86517367..86517386
60.32
50
downstream
ENSMUSE00000356356
Chr11:86517166..86517276
TGGTTCTGCACTGAGCGTAA
Chr11:86517205..86517224
60.6
50
downstream
ENSMUSE00000398849
Chr11:86516103..86516273
CAAACGCTGAGCAAGTGAGA
Chr11:86516189..86516208
60.32
50
downstream
ENSMUSE00000357846
Chr11:86515711..86515932
CTGGATGAAATAGGGCATGG
Chr11:86515713..86515732
60.29
50
downstream
ENSMUSE00000649327
Chr11:86514563..86514583
No primer for this exon
downstream
ENSMUSE00000373572
Chr11:86510767..86510842
AGCGACTCCGAAGCATCTAA
Chr11:86510792..86510811
60.12
50
downstream
ENSMUSE00000705983
Chr11:86509068..86509192
CCCATACGGTGGTGCAGTAT
Chr11:86509079..86509098
60.66
55
downstream
ENSMUSE00000439707
Chr11:86508161..86509192
AGAACACGTTGCCTGTTTCC
Chr11:86508953..86508972
60.16
50
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
AAATAATCGCCTTGCAGCAC
Chr11:86552927..86552947
60.24
45
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
ATCCAAACGTGACTGGGAAA
Chr11:86552931..86552951
60.35
45
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000047126