Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI29582
Trapped Gene
Ncstn (ENSMUSG00000003458)
Vector Insertion
Chr 1: 174011342 - 174012846
Public Clones (sanger) E324H06 (ggtc) E323E05 (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 4% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000378092 (Chr1:174012732..174012845 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000378092 (Chr1:174012732..174012845 -)
Downstram Exon
ENSMUSE00000160455 (Chr1:174011343..174011447 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000378092 Chr1:174012732..174012845 No primer for this exon
upstream ENSMUSE00000160455 Chr1:174011343..174011447 No primer for this exon

*** Putative Vector Insertion (Chr 1: 174011342 - 174012846) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000160445 Chr1:174005536..174005659 No primer for this exon
downstream ENSMUSE00000160453 Chr1:174005046..174005167 No primer for this exon
downstream ENSMUSE00000160454 Chr1:174004404..174004549 No primer for this exon
downstream ENSMUSE00000160446 Chr1:174002908..174003058 No primer for this exon
downstream ENSMUSE00000160444 Chr1:174002597..174002706 No primer for this exon
downstream ENSMUSE00000160457 Chr1:174002252..174002404 No primer for this exon
downstream ENSMUSE00000160452 Chr1:174001596..174001700 No primer for this exon
downstream ENSMUSE00000160456 Chr1:174001382..174001459 No primer for this exon
downstream ENSMUSE00000160443 Chr1:174000107..174000279 No primer for this exon
downstream ENSMUSE00000160451 Chr1:173998893..173998995 No primer for this exon
downstream ENSMUSE00000160450 Chr1:173998693..173998788 No primer for this exon
downstream ENSMUSE00000160442 Chr1:173998317..173998404 No primer for this exon
downstream ENSMUSE00000160448 Chr1:173997920..173998074 No primer for this exon
downstream ENSMUSE00000160447 Chr1:173997462..173997674 No primer for this exon
downstream ENSMUSE00000384073 Chr1:173996805..173996927 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
  No PCR available

Come back to gene ENSMUSG00000003458