Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI29647
Trapped Gene
Rnf185 (ENSMUSG00000020448)
Vector Insertion
Chr 11: 3338207 - 3352310
Public Clones (sanger) E127G06 (ggtc) IST11690H1 (tigm) IST14487A4 (tigm) IST10332D12 (tigm)
IST11690H1 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000416051 (Chr11:3352212..3352309 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000416051 (Chr11:3352212..3352309 -)
Downstram Exon
ENSMUSE00000416041 (Chr11:3338208..3338326 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000416051 Chr11:3352212..3352309 No primer for this exon
upstream ENSMUSE00000595379 Chr11:3352212..3352366 No primer for this exon
upstream ENSMUSE00000416041 Chr11:3338208..3338326 No primer for this exon
upstream ENSMUSE00000682440 Chr11:3338208..3338270 No primer for this exon

*** Putative Vector Insertion (Chr 11: 3338207 - 3352310) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000581549 Chr11:3332403..3332623 No primer for this exon
downstream ENSMUSE00000581554 Chr11:3332403..3332623 No primer for this exon
downstream ENSMUSE00000656344 Chr11:3328887..3328905 No primer for this exon
downstream ENSMUSE00000104745 Chr11:3326569..3326681 No primer for this exon
downstream ENSMUSE00000104747 Chr11:3325285..3325339 No primer for this exon
downstream ENSMUSE00000104743 Chr11:3319865..3319982 No primer for this exon
downstream ENSMUSE00000332999 Chr11:3315986..3318090 No primer for this exon
downstream ENSMUSE00000706248 Chr11:3315985..3318090 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CACCCAAGCACAAGTCACTG Chr11:3352265..3352285 60.35 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CACCCAAGCACAAGTCACTG Chr11:3352265..3352285 60.35 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000020448