Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI2968
Trapped Gene
Rassf1 (ENSMUSG00000010067)
Vector Insertion
Chr 9: 107457257 - 107458941
Public Clones AB0333 (sanger) IST14980A11 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000254340 (Chr9:107457090..107457256 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000254340 (Chr9:107457090..107457256 +)
Downstram Exon
ENSMUSE00000710890 (Chr9:107458942..107459082 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000716703 Chr9:107453898..107454177 No primer for this exon
upstream ENSMUSE00000583263 Chr9:107453928..107454177 No primer for this exon
upstream ENSMUSE00000720513 Chr9:107456457..107456575 No primer for this exon
upstream ENSMUSE00000583262 Chr9:107456469..107456575 No primer for this exon
upstream ENSMUSE00000254340 Chr9:107457090..107457256 No primer for this exon

*** Putative Vector Insertion (Chr 9: 107457257 - 107458941) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000710890 Chr9:107458942..107459082 No primer for this exon
downstream ENSMUSE00000221062 Chr9:107459784..107459888 No primer for this exon
downstream ENSMUSE00000221059 Chr9:107460101..107460398 No primer for this exon
downstream ENSMUSE00000221063 Chr9:107460518..107460633 No primer for this exon
downstream ENSMUSE00000254319 Chr9:107463730..107464591 No primer for this exon
downstream ENSMUSE00000720089 Chr9:107463730..107464594 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CTAATCGCCTTGCAGCACAT Chr9:107457307..107457327 61.32 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CTGGAACCGTGACTGGGAAA Chr9:107457301..107457321 63.35 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000010067